宫颈癌及其癌前病变组织NET-1基因蛋白的表达及意义

目的:探讨宫颈癌及其癌前病变组织中NET-1基因蛋白的表达及意义。方法:应用免疫组化S-P法对50例宫颈鳞癌组织、36例宫颈上皮内瘤样病变(CINⅠ～Ⅲ级)及5例正常宫颈组织中的NET-1基因蛋白进行检测。结果:NET-1基因蛋白在正常宫颈组织中不表达,在CINⅢ中表达显著高于CINⅠ和CINⅡ(均P<0.01);在CINⅢ和宫颈癌中的表达阳性率分别为78.57%、76.00%,两组比较无显著差异(P>0.05);NET-1表达阳性率及阳性强度与宫颈癌淋巴结转移呈显著正相关(P<0.01)。结论:NET-1过表达可能是宫颈癌发生的早期分子事件,可作为CIN病情监测、处理的辅助指标;NET-1高表达赋予了癌细胞更显著的侵袭、转移潜能。

Expression of NET- 1 gene protein and its significance in cervical cancer and its precursor lesion.

Objective:To study the expression of NET-1 gene protein and its significance in cervical cancer and its precursor lesion.Methods:Immunohistochemical S-P method was used to detect NET-1 gene protein in 50 cases of cervical invasive squamous cell carcinoma,36 cases of cervical intraepithelial neoplasia(CINⅠ～Ⅲ)and 5 cases of normal cervical tissue.Results:There was no expression of NET-1 in normal cervical tissues.The positive expression rate of NET-1 in CINⅢ was significantly higher than that in CINⅠ(P<0.01)and CINⅡ(P<0.01).The positive expression rate of NET-1 in CINⅢ and cervical cancer was 78.58% and 76.00% respectively,and the difference was not significant(P>0.05).The expression of NET-1 was closely correlated with lymph node metastasis in cervical cancer(P<0.01).Conclusion:The overexpression of NET-1 may be an early molecule event in the occurrence of cervical cancer and become a marker to monitor and predicate the transit of CIN in clinical practice.The overexpression of NET-1 contributes apparent potential abilities of invasion and metastasis to carcinoma cells.