不同C6细胞种植量对大鼠生存期及脑干胶质瘤生长的影响

目的 观察不同C6细胞数量种植于大鼠脑干部位对大鼠生存期及肿瘤生长速度的影响.方法 30只Wistar大鼠随机分成三组,分别将2×104(A组)、1×106(B组)个C6胶质瘤细胞和等量生理盐水(C组)注射到大鼠桥脑,种植后1、2、3和4周分别通过行为学、生存期、MRI及病理HE染色观察各组之间的差异.结果 A组大鼠接种后16 d出现行为学异常,而B组大鼠则于第9天出现.A组大鼠平均生存期为(30.80±3.002)d,B组为(19.65±2.277)d.MRI检查显示,接种后2周B组大鼠脑干肿瘤的体积明显大于A组(P＜0.05).HE染色显示两组肿瘤特点无差异.结论 种植2×104和1×106个细胞量均可成瘤,但接种细胞数量越多成瘤越快,生存期越短.提示我们在制模过程中,应根据具体的实验要求选用不同的细胞数量.

Effect on rat survival and tumor growth by different implanting number of C6 cell

Objective To investigate the effect of different number C6 cell implanted to brainstem on rat survival time and tumor growth.Methods 30 Wistar rats were divided into 3 groups randomly,injected with 2×104( group A),1×106( group B) C6 glioma cells and physiological saline (group C) into rat brainstem respectively.1,2,3,4 weeks later rat signs,survival time,tumor size by MRI and pathology by H&E were compared.Results Group A emerged abnormality at day 16,but group B at day 9.Group A' s survival time was 30.80±3.002 days,group B was 19.65±2.277 days.Comparing with tumor size,group B was remarkably larger than group A (P＜0.05).H&E showed no difference.Conclusions Both number of 2×104 and 1×106 C6 cells can make a tumor model,but more tumor cells injecting causes a earlier tumor formation and shorter survival time.