HD-A+VM_(26)+rhG-CSF动员白血病患儿外周血造血干细胞的初步研究

目的 　评价HD -A +VM2 6+rhG -CSF动员白血病患儿外周血造血干细胞的效果。方法 　 5例高危急性白血病患儿 ,其中急性淋巴细胞白血病 (HR -ALL) 4例 ,急性杂合细胞白血病 (AHL) 1例。年龄 9～ 1 3岁。 5例患儿均采用HD -A +VM2 6+rhG -CSF方案进行动员。在化疗后第 9天应用rhG -CSF ,当白细胞升至 5× 1 0 9/L以上时 ,使用CS - 30 0 0血细胞分离机采集PBSC。结果 　 4例采集 1次 ,1例采集 2次。全部患儿均能耐受该动员方案治疗。MNC值为 5 96 5( 5 0 2～ 7 2 )× 1 0 8/kg。CD3 4+ 细胞量为 4 372 ( 3 95～ 5 6 2 )× 1 0 6/kg。结论 　G -CSF联合大剂量Ara -C和VM2 6的化疗可采集到足够数量的自体外周血干细胞。

The stem cell mobilizing capacity of HD-A+VM26+rhG-CSF for childhood Leukemia

Objective To investigate the clinical value of rh-G-CSF combined with high-dose Ara-C and VM 26for mobilizing peripheral blood stem cell in children with acute leukemia. Methods 5 patients with acute leukemia accepted rh-G-CSF combined with high-dose Ara-C and VM 26for mobilizing peripheral blood stem cell. The age was 9～13 years old. Four case of high-rish acute lymphoblastic leukemia (HR-All), 1 case of hybrid acute leukemia(HAL). G-CSF was given on day 9 after chemotherapy. When WBC>5×109/L,APBSC were harvested with CS 3000 plus Spectra. Results Four patients were collected one time, one patient two times. All of the patient could tolerate the treatment regimens. The harvested MNC was 5.965(5.02～7.2)×108/kg. CD 34+ cell was 4.372(3.95～5.62)×106/kg. Conclusion rh-G-CSF combined with high-dose Ara-C and VM 26is an optimal method for APBSC mobilization in acute leukemia in childood.