| 小鼠白细胞介素18真核表达质粒的构建及基因多态性分析 |
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| 引用本文： | 时阳,谭岩,刘力华,太京华,方艳秋,段秀梅,姜艳芳,许淑芬. 小鼠白细胞介素18真核表达质粒的构建及基因多态性分析[J]. 吉林大学学报(医学版), 2004, 30(2): 206-208. DOI: 卫生部临床学科重点科研项目；吉林省科技厅 |
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| 作者姓名： | 时阳 谭岩 刘力华 太京华 方艳秋 段秀梅 姜艳芳 许淑芬 |
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| 作者单位： | 吉林大学第一医院中心实验室，吉林 长春130021 |
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| 基金项目： | 卫生部临床学科重点项目 , 吉林省科技厅科研项目 , 吉林省卫生厅科研项目 , 吉林大学校科研和教改项目 , 吉林大学校科研和教改项目 |
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| 摘 要： | 目的：构建小鼠白细胞介素18(mIL-18)的重组真核表达质粒，制备有效的免疫佐剂。方法：通过RT-PCR分别从BALB/c小鼠和Swiss小鼠肝组织总RNA中扩增mIL-18，并将其连接于载体pcDNA3中，再用双酶切及测序方法鉴定重组质粒。结果：构建出两组重组质粒，其中从BALB/c小鼠中获得的mIL-18序列与Genbank中序列完全一致，而从Swiss小鼠中获得的mIL-18序列有两个点突变。结论：重组质粒pcIL-18构建成功，且发现mIL-18具有未见报道的点突变，已登录于Genbank。这为研制抗肿瘤DNA疫苗，增强疫苗疗效奠定基础。
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| 关 键 词： | 真核表达质粒 佐剂 免疫 多态现象(遗传学) |
| 文章编号： | 1671-587X(2004)02-0206-03 |
| 收稿时间： | 2003-11-11 |
| 修稿时间： | 2003-11-11 |
Construction of recombinant eukaryotic expressing plasmid of mouse IL-18 and analysis of its gene polymorphism |
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| SHI Yang,TAN Yan,LIU Li-hua,TAI Jing-hua,FANG Yan-qiu,DUAN Xiu-mei,JIANG Yan-fang,XU Shu-fen. Construction of recombinant eukaryotic expressing plasmid of mouse IL-18 and analysis of its gene polymorphism[J]. Journal of Jilin University: Med Ed, 2004, 30(2): 206-208. DOI: 卫生部临床学科重点科研项目；吉林省科技厅 |
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| Authors: | SHI Yang TAN Yan LIU Li-hua TAI Jing-hua FANG Yan-qiu DUAN Xiu-mei JIANG Yan-fang XU Shu-fen |
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| Affiliation: | Central Laboratory, First Hospital, Jilin University, Changchun 130021, China |
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| Abstract: | ObjectiveTo construct recombinant Murine IL 18 (mIL 18) plasmid which could be expressed in eukaryotic systems and be used as immunoadjuvant. MethodsmIL 18 was amplified from BALB/c and Swiss mouse liver mRNA by RT PCR, and ligated orientatively into the eukaryotic expressing plasmid pcDNA3. The recombinant plasmids, pcIL 18,were evaluated with endorebonuclease digestion and sequencing. ResultsTwo recombinant plasmids were constructed. The sequence of mIL 18 from BALB/c mouse was consistent with that of Genbank, while the one from Swiss mouse had two point mutations. ConclusionTwo recombinant plasmids pcIL 18 are constructed successfully. They will play a key role in reinforcing DNA vaccine. And gene polymophism in mIL 18 has been found and received by Genbank (AY362457). |
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| Keywords: | interleukin 18 eukaryotic expressing plasmid adjuvarits immunologic polymorphism (genetics) |
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