| 新疆中麻黄基因组DNA文库的构建 |
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| 引用本文： | 马永红,党荣理,叶志远,吴霞,刘砥威,刘庆华.新疆中麻黄基因组DNA文库的构建[J].医学动物防制,2005,21(4):300-303. |
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| 作者姓名： | 马永红 党荣理 叶志远 吴霞 刘砥威 刘庆华 |
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| 作者单位： | 1. 新疆军区卫生防疫大队,乌鲁木齐,830002
2. 新疆药物研究所,乌鲁木齐,830000 |
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| 摘 要： | 目的:构建新疆中麻黄(EphedraIntermedia)的基因组DNA文库。方法用Sau3AI对所提新疆中麻黄基因组DNA进行酶切,回收15-23Kb之间的酶切产物片段,然后与LambdavectorBamHIArms进行连接、包装形成文库,计算文库重组噬菌体滴度、包装效率。结果,所建文库的重组噬菌体滴度为1×106pfu/ml、包装效率为2×106重组子/μg载体DNA。结论成功构建了基因组DNA文库,为进一步研究奠定了基础。
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| 关 键 词： | 中麻黄 基因组DNA文库 构建 新疆 |
Construction of the genomic DNA library in Ephedra intermedia in sinkiang |
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| MA Yong-hong,DANG Rong-li,YE Zhi-Yuan,Wu xia,LIU Di-wei,LIU Qing-hua.Construction of the genomic DNA library in Ephedra intermedia in sinkiang[J].Chinese Journal of Pest Control,2005,21(4):300-303. |
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| Authors: | MA Yong-hong DANG Rong-li YE Zhi-Yuan Wu xia LIU Di-wei LIU Qing-hua |
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| Institution: | MA Yong-hong1,DANG Rong-li1,YE Zhi-Yuan1,WU Xia2,LIU Di-wei2. LIU Qing-hua2 |
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| Abstract: | Objective: The aim is to construct the genomic DNA library in Ephedra intermedia in Sinkiang.METHODS The genomic DNA from Ephedra intermedia in Sinkiang was partially digested with Sau3AI.The large-scale digestiong products which contain 15-23kb size range of fragments were separated by electrophoresis. Suitable molecular ratio of the separated fragments to Lambda vector BamH I Arms was choiced to proceed the ligation by T4 DNA ligase. The ligated DNA was added to a Packagene Extract, and the packaged phage was formed. The titer of the phage and the packaging efficiency of arms were calculated. RESULTS The titer of the phage was 1×1 6 pfu/ml and the packaging efficiency of arms was 2×10 6 recombinants/μg DNA. CONCLUSION The results demonstrate that the genomic DNA library has been successfully constructed in Ephedra intermedia in Sinkiang |
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| Keywords: | Ephedra intermedia genomic DNA library construction Sinkiang |
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