不同浓度锂对大鼠原代培养大脑皮层神经元的影响

目的　通过研究不同浓度锂对原代培养的皮层神经元生长及存活的影响 ,进一步探讨锂的神经元保护作用以及锂对脑发育的影响。方法　建立新生大鼠皮层神经元原代培养技术 ,通过加载 0 .6 2 5、1.2 5 0、2 .5 0 0、5 .0 0 0和 10 .0 0 0mmol L浓度的氯化锂 ,镜下观察神经元的生长和存活情况 ,测量神经元最长突起长度 ,通过噻唑蓝 (MTT)还原法进一步观察细胞的生长活力。结果　与对照组相比 ,氯化锂处理组的神经元胞体透亮 ,出芽快 ,神经元突起明显增长 ;且生长活力强于对照组 ,以5 .0 0 0mmol L浓度 (5 3.80± 5 .84 ) μm]的锂作用最强 ,差异有显著性 (P <0 .0 1)。结论　锂可促进原代培养皮层神经元的生长和存活。

Effects of lithium on primary cultured cerebrocortical neurons of rat

OBJECTIVE: To explore the neuroprotection and the impact on brain development of lithium, the effects of lithium salt on the growth and survival of primary cultured cerebrocortical neurons were studied. METHODS: The technique of primary cultured cerebrocortical neurons of newborn rats with serum-free medium was established, and the growth and survival of neurons treated with different doses of lithium chloride (0.625, 1.250, 2.500, 5.000, 10.000 mmol/L) were observed. The length of neuronal synapse, cell viability by MTT reduction assay were also measured. RESULTS: The neurons were brighter, germinated rapidly, the neuronal synapse lengthened markedly, and the neurons viability was also better after treated with lithium chloride. Among the five doses, 5.000 mmol/L had the best effect (53.80 +/- 5.84) micro m, P < 0.01]. CONCLUSION: Lithium chloride can promote the growth and survival of neurons.