微柱凝胶法和凝聚胺法在维持性血透患者交叉配血中的应用分析

目的:比较首次输血和多次输血后不规则抗体检测的阳性率,探讨微柱凝胶法(microcolumn gel test,MGT)和凝聚胺法(manual polybrene test,MPT)在维持性血透患者交叉配血中的应用价值。方法:采用MGT和MPT对需输血的96例维持性血透患者进行交叉配血试验。结果:96例血透患者462例次交叉配血中,MGT法和MPT法配血相合差异有统计学意义(P<0.01)。其中MGT主侧次侧凝集62例直接抗人球蛋白试验全部阳性。并且随着输血的次数增加,MGT和MPT不规则抗体的阳性率均增加(P<0.01)。MGT检测总阳性率高于MPT(P<0.01)。结论:维持性血透患者长期、多次输血后,会出现抗体的不规则性。在MGT中致敏红细胞会导致配血困难。结合MPT进一步了解凝集的性质,可以提高输血的安全性。在MGT交叉配血次侧阳性的情况下,同时做MPT交叉配血具有很好的互补性。

Analysis on application in the crossmatching of sustainable hemodialysis' patients by microcolumn gel test and manual polybrene test

Objective:To compare irregular antibodies′ postive rates of the first and repeated blood transfusions,and to approach applied value of the crossmatching test by microcolumn gel test(MGT)and manual polybrene test(MPT)in the sustainable hemtodialysis′ patients.Methods:Ninety-six patients sustainable hematodialysis′ who required blood transfusion were crossmatched by MGT and MPT.Results:In 462 cross matching tests for 96 patients,the difference of crossmatching compatibility showed statistical significance between MGT and MPT(P<0.01).Among the total,62 cases of MGT major and minor incompatibility were positive by direct antiglobulin test.Moreover,with the increase of transfusion′times,the positive rate of irregular antibodies also increased by MGT and MPT(P<0.01).The total positive rate was higher using MGT than using MPT,the susceptibility of MGT was obviously higher than MPT(P<0.01).Conclusions:In sustainable hemtodialysis′patients,there will be line irregularity of antibodies after continual and repeated transfusions.In MGT,sensitized RBC will lead to difficulty of crossmatching test.In combination with MPT,we can further understand the nature of incompatibility,which can improve safety of transfusion.It might be of good complementarity when there is minor incompatibility of MGT in cross matching.