大鼠类风湿性关节炎动物模型制备及血沉和组织学检测

目的:探讨大鼠类风湿性关节炎(RA)动物模型的制备及血沉和组织学检测。方法:以实验大白鼠为对象,Ⅱ型胶原(CⅡ)和不完全弗氏佐剂(IFA)混合注射,不同时间点实验组大鼠(0、2、4、6周)所获得血样用于做血沉检测,踝关节标本进行组织病理学研究。结果:实验组与正常对照组血沉指标进行统计学比较分析,差异有统计学意义(P<0.05),而且在急性期增快明显,病情稳定后血沉下降。在大鼠关节炎标本组织学中观察到滑膜组织增生,其局部骨吸收破坏明显,软骨表层胶元纤维松解断裂、软骨细胞变性坏死,软骨表面凸凹不平,可见骨吸收破坏陷窝。结论:采用异种Ⅱ型胶原蛋白与弗氏完全佐剂可以成功制备的类风湿关节炎模型,可以用于RA炎性吸收破坏机理及治疗方面的研究。

The establishment of rat rheumatoid arthritis model to observe ESR and pathological changes

Objective By injecting the collagen protein Ⅱ and Freund's incompleted adjuvant,to establish rat rheumatoid arthritis animal model.Method The animal model was established by injecting Freund's incomplete adjuvant into rats.The histopathological changes of the rats' ankle joint and erythrocyte sedimentation rate(ESR) were studied respectively at the 2 th,4 th,6th weeks after the animal models for joint had been established.Results Assayed the erthrocyte sedimentation rate at various time points,the values were increased obviously at acute phase,compared with normal group(P<0.01),and decrease gradual was emerged when it turned into chronic phase.Inflammation was presented in the ankle joint specimens,which included proliferation of the synovium,erosion into the articular cartilage and bone.The surface of the joint cartilages were rough,fibro of collagen broken and cartilage cell necrosis,the pits because of bone destroyed could be observed.Conclusion By injecting the collagen protein Ⅱ and Freund's incompleted adjuvant,we can get the animal models of rat ankles rheumatoid arthritis(RA),which histological and clinical changes are similar with that of human,so it can be used to study mechanism of RA and treatmeat of the RA.