抑制 HOTAIR 的表达促进替莫唑胺引起的垂体腺瘤细胞凋亡

目的：探索长链非编码RNA（long noncoding RNA ，lncRNA）HOTAIR在替莫唑胺引起的垂体瘤细胞凋亡中的功能。方法体外培养垂体腺瘤细胞系 HP75细胞和GH3细胞，使用替莫唑胺处理 HP75细胞和GH3细胞后，采用实时定量PCR法检测细胞中 HOTAIR的表达；采用RNA干扰（RNA interference ，RNAi）技术敲低内源性 HOTAIR的表达；并应用MTT法检测敲低内源性HOTAIR后细胞增殖能力的改变，应用流式细胞术检测敲低内源性 HOTAIR后替莫唑胺对垂体腺瘤细胞凋亡的影响。结果替莫唑胺能够引起垂体腺瘤HP75细胞和GH3细胞显著的凋亡，并且在此过程中HOTAIR的表达显著降低；采用RNAi技术能够显著地抑制垂体腺瘤细胞系中HOTAIR的表达；敲低HOTAIR后，细胞的增殖能力受到明显的抑制，如果同时使用替莫唑胺处理细胞，细胞的凋亡率出现显著地升高。结论敲低内源性 HOTAIR的表达能够促进替莫唑胺引起的垂体腺瘤细胞凋亡，二者具有协同作用。

Silencing of HOTAIR expression inhibits the temozolomide-induced apoptosis in pituitary tumor cells

Objective To investigate the effect of long non-coding RNA (lncRNA) HOTAIR on the temozolomide-induced apoptosis of pituitary tumor cells .Methods Pituitary adenoma cell line HP75 cells and GH3 cells were cultured in vitro ,quanti-tative real-time PCR was used to detect the expression level of lncRNA HOTAIR in HP75 cells and GH3 cells after treatment with temozolomide .The HP75 cells and GH3 cells were transfected with siRNA specially targeting HOTAIR to knock endoge-nous HOTAIR down in vitro ;furthermore ,MTT assay was used to detect the proliferation rate in HP75 and GH3 cells ,flow cytometry assay was employed to detect the temozolomide-induced apoptosis in HP75 cells and GH3 cells .Results Temozolo-mide could cause significant apoptosis in pituitary tumor HP75 cells GH3 cells ,at the same time ,the expression of HOTAIR was significantly reduced in cells treated with temozolomide;the expression of HOTAIR could be significantly inhibited via transfecting with siRNA targeting HOTAIR in HP75 cells and GH3 cells ;the knockdown endogenous HOTAIR could inhibit the proliferation rate in HP75 cells and GH3 cells ,and enhance the apoptosis rate in HP75 cells and GH3 cells treated with te-mozolomide .Conclusion Interference on the expression of HOTAIR in pituitary tumor cells could enhance the temozolomide-induced apoptosis of pituitary adenoma cells .