Reaktive träger zur immobilisierung von enzymen ausgehend von polyacrolein

Polyacrolein ( 1 ) was transformed into poly(allyl alcohol) ( 2 ), poly1,1-bis(hydroxymethyl)ethylene] ( 5 ) and N-substituted poly(vinylmethanimine)s 8, 9a–c and 10 . By subsequent reactions on these polymers reactive carriers with isothiocyanato-, diazo-, and azido groups were formed or by reaction with BrCN activated carriers were obtained which are able to bind enzymes covalently. The binding abilities of some reactive carriers were investigated for papain and trypsin. The effect of the pH-value during the coupling reaction, the amount of offered enzyme, the content of the reactive groups per g carrier and the effect of different reactive groups on the binding ability of the carriers were investigated. For immobilized papain and trypsin the enzymatic activity, the pH-optimum of their activity, and the apparent Michaelis constant K_M(app) were determined. The dependence of the activity on the temperature was also investigated.