Experimental infection of chickens with Histomonas meleagridis confirms the presence of antibodies in different parts of the intestine

Specific pathogen-free chickens were infected with a clonal culture of the protozoan parasite, Histomonas meleagridis. Severe lesions were found within the caeca of all birds euthanized at 7 and 14 days post-infection (d.p.i.). Following necropsy of birds, intestinal samples were taken to establish ex vivo tissue cultures to determine the IgG, IgA and IgM antibody levels in the supernatants before and after incubation with a recently established ELISA. Presence of antibodies was also determined in the sera and first optical density values for IgG above the cut-off were detected at 14 d.p.i. IgA levels remained low in the serum with a small peak 4 weeks p.i., a phenomenon also found for IgM. The intestinal tissue samples showed very strong immunological reactions in the parasitized caeca with an initial peak of IgM, high levels of IgG and a continuous increase of IgA. In the duodena and jejuna, IgA values reached similar high levels as those obtained in the caeca, whereas IgG and IgM increased only slightly.