| 套式PCR检测滤纸干血滴中间日疟原虫 |
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| 引用本文： | 牛春,诸欣平,周蕾,刘强.套式PCR检测滤纸干血滴中间日疟原虫[J].寄生虫与医学昆虫学报,2000,7(1):7-10. |
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| 作者姓名： | 牛春 诸欣平 周蕾 刘强 |
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| 作者单位： | 首都医科大学细胞生物系寄生虫学教研室,北京,100054 |
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| 基金项目： | 教育部留学回国人员科研启动基金，北京市教委科技发展计划项目 |
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| 摘 要： | 从滤纸干血滴上用Ｃｈｅｌｅｘ处理洗脱下的疟原虫ＤＮＡ，经套式ＰＣＲ扩增间日疟虫ＳＳＵｒＲＮＡ基因特异性１２１ｂｐ片段，分析该方法的敏感性和特异性。３７例血栓检测结果一部阳性，当原虫密度低全２５个原虫／ｕＬ血时仍可成功检测到该特异条带，且其它三种人疟原虫（恶性疟虫，三日疟原虫和卵疟原虫）血样均为阴性。提示滤纸干血滴与ＰＣＲ扩增技术相结合，是疟疾诊断或流行病学调查的实用工具。
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| 关 键 词： | 间日疟原虫 套式PCR 滤纸于血滴 Chelex 疟疾 |
DETECTION OF PLASMODIUM VIVAX BY NESTED PCR AMPLIFICATION OF DRIED BLOOD SPOTS ON FILTER PAPERS |
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| Niu Chun,Zhu Xinping,Zhou Lei,Liu Qiang.DETECTION OF PLASMODIUM VIVAX BY NESTED PCR AMPLIFICATION OF DRIED BLOOD SPOTS ON FILTER PAPERS[J].Acta Parasitologica et Medica Entomologica Sinica,2000,7(1):7-10. |
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| Authors: | Niu Chun Zhu Xinping Zhou Lei Liu Qiang |
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| Abstract: | Malaria parasites DNA was eluted from dried blood spot on filter paper by Chelex treatment and amplified by PCR to detect low parasitemia of Plasmodium vivax. Sensitivity and specificity of nested PCR assay were confirmed by amplifying a 121 bp DNA fragment of SSUrRNA gene of Plvivax.Density of about 25 parasites per ul of blood can be detected successfully.None of other three human malaria species(Plasmodium falciparum,Plasmodium malariae and Plasmodium ovale)samples was positive.The ease of collection and transport of filter paper specimens combined with the sensitive and specific detection of P.vivax by nested PCR suggest that this method might be a valuable tool for moleculr epidemiological study of P.vivax. |
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| Keywords: | Chelex Plasmodium vivax nested-PCR blood filter paper Chelex |
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