表皮葡萄球菌体外与纤维蛋白原的结合力研究

目的 :比较纤维蛋白原结合基因 (fbe)阳性和纤维蛋白原结合基因阴性的表皮葡萄球菌 (表葡菌 )体外与纤维蛋白原结合力的差异 ,探讨 fbe基因在表葡菌致异物感染中的致病作用。 方法 :采用ATB鉴定临床分离菌 ,聚合酶链反应 (PCR)方法检测有无 fbe基因 ,采用酶联免疫吸附法 (ELISA)测定并比较 fbe基因阳性和 fbe基因阴性表葡菌体外与纤维蛋白原的结合力。结果 :6 2株临床分离菌中有 4 6株为表葡菌 ,表葡菌中 fbe基因阳性的菌株为 34株 ,阳性率为 73.9% ,其他葡萄球菌均不含该基因 ;fbe基因阳性菌株和fbe基因阴性菌株体外与纤维蛋白原的结合力差异有显著性 (P <0 .0 1 )。 结论 :fbe基因阳性菌株和fbe基因阴性菌株体外与纤维蛋白原的结合力存在差异 ,提示 fbe基因可能为表葡菌致异物感染的致病因素之一。

In vitro study on adhesion of Staphylococcus epidermidis to fibrinogen

Objective: To explore the pathogenicity of fbe gene (fibrinogen binding gene) of S. epidermidis in foreign bodies associated infections via comparing the difference in adhesion to fibrinogen (Fg) between fbe gene positive and fbe negative S. epidermidis strains in vitro. Methods: ATB system was used to identify clinical strains, fbe in strains was detected by PCR and the adhesion of fbe (+) and fbe (-) S. epidermidis strains to Fg were detected by an ELISA method. Results: 46 of 62 clinical isolates of staphylococci were S. epidermidis strains, 34 of which were fbe gene positive accounted for 73.9%. There was significant difference in adhesion to Fg between fbe gene positive and negative strains of S. epidermidis in vitro ( P < 0.01). Conclusions: The significant difference of adhesion to Fg between fbe gene positive and fbe negative strains suggest that fbe gene maybe one of the potential virulence factors of S. epidermidis in foreign bodies associated infections.