脑梗死患者体液S-100b蛋白含量测定及其临床意义

目的　为了观察急性和恢复期脑梗死患者血液和脑脊液的S 10 0b蛋白含量变化 ,探讨其与神经功能缺损程度、脑梗死体积以及患者年龄等方面的相关性。方法　将 4 6例脑梗死住院患者作为观察组 ,2 5例与观察组相匹配的骨折、阑尾或胆囊摘除手术病人以及健康体检者作为对照组 ;采用双抗体夹心ELISA法 ,检测其血液及脑脊液S 10 0b蛋白的含量 ;并应用SPSS10 .0统计软件包进行统计学分析。结果　急性脑梗死 (ACI)患者血液和脑脊液S 10 0b蛋白显著高于恢复期患者和对照组 (P <0 .0 1) ;血液和脑脊液S 10 0b蛋白含量与梗死体积均呈显著正相关 (P <0 .0 1) ;与出院时神经功能缺损程度呈显著正相关 (P <0 .0 1)。结论　脑脊液或血清中的S 10 0b蛋白浓度 ,反映了神经胶质细胞的损害程度 ,也是脑组织破坏后较合适的生化标记物 ,有助于判断脑梗死病人梗死范围、监测病情变化、疗效观察。

Clinical significance of serum and cerebrospinal fluid S-100b protein in patients with cerebral infarction

Objective To evaluate the clinical significance of S-100b protein in serum and cerebrospinal fluid of patients with acute and recovery cerebral infarction and to find its correlation with neurologic deficit s, infarct volume and ages. Methods 46 patients with cere bral infarction in test group and 25 in control group were measured for S-100b protein of cerebrospinal fluid and serum by enzyme-linked immunosorbent assay. Comparison was performed by SPSS10.0 statistical package. Results Patients with acute cerebral infarction has a higher concentration of S -100b protein of serum and cerebrospinal fluid than in control group( P <0. 01). The release of S-100b protein into serum and cerebrospinal fluid in patie nts with acute cerebral infarction is associated with the neurological outcome at discharge from hospital as assessed by Chinese stroke scale scores( P <0. 01)and the volume of brain lesions( P <0.01)in acute cerebral infarction( P <0.01). Conclusion Our results show that the conce ntration of S-100b protein in serum and cerebrospinal fluid of patients with ac ute cerebral infarction imply the acquity of the disease or the extent of lesion of nueroglial cell.