不同抵抗素水平对血清游离脂肪酸的影响

目的 观察内源性与外源性不同浓度抵抗素水平对大鼠血清游离脂肪酸(FFA)的影响.方法 通过尾静脉注入重组大鼠抵抗素制作外源性高抵抗素大鼠模型,将大鼠分为抵抗素组(RE组,n=8)和生理盐水对照组(NS组,n=8).饮食诱导并筛选出内源性高抵抗素大鼠模型,将大鼠分为高抵抗素组(HRE组,n=8)、正常抵抗素组(NRE组,n=8)和正常对照组(NC组,n=8),观察不同抵抗素水平下FFA浓度的变化.结果 RE组大鼠血清FFA、胰岛素水平均显著高于NS组(P<0.01).HRE组大鼠FFA水平较NRE组和NC组均显著升高(P<0.01).血清抵抗素与FFA、胰岛素、血糖、胰岛素敏感指数(ISI)、体质量、Lee指数均显著相关(P<0.01).控制胰岛素、血糖、ISI、体质量、Lee指数等混杂因素后,血清抵抗素仍然与FFA显著相关(r=0.615, P<0.01).结论 外源性与内源性抵抗素升高均可引起血清FFA升高;高抵抗素血症在脂代谢紊乱中发挥重要作用.

Effect of resistin with different levels on serum free fatty acid

Objective To elucidate the effects of resistin with different levels on serum free fatty acid (FFA) in intrinsic and extrinsic hyper-resistin models. Methods The extrinsic hyper-resistin models were established by infusion of rat recombinant resistin through the tail vein and defined as resistin group (RE group, n=8); the normal saline group (NS group, n=8) was defined as control. After high-fat diet, the rats with resistin levels higher than X^-±2s of resistin were defined as diet-induced hyper-resistinemia model group (HRE group, n=8) and those with resistin levels lower than X^-±s of resistin were defined as diet-induced normal resistin model group (NRE group, n=8); the normal control group (NC group, n=8) received standard feed at the same time. Concentrations of FFA with different resistin levels were detected. Results The levels of the serum FFA and insulin in RE group was higher than those in NS group (P<0.01). The level of FFA increased in HRE group than that in NRE group and NC group (P<0.01). Resistin levels were obviously correlated with serum FFA, insulin, glucose, insulin sensitivity index (ISI), weight and Lee index (P<0.01). Even after correction for insulin, glucose, ISI, weight and Lee index, the resistin level was still correlated significantly with FFA (r=0.615, P<0.01). Conclusion Both extrinsic and intrinsic hyper-resistin can induce higher FFA levels in rats. The hyper-resistin in rats may play an important role in lipid metabolism disorder.