| 远端缺血预处理在小体积肝移植物缺血-再灌注损伤的保护作用 |
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| 引用本文： | 桂亮,张斌,沈健,唐劲草,王猛,封冰,李相成.远端缺血预处理在小体积肝移植物缺血-再灌注损伤的保护作用[J].江苏医药,2011,37(11). |
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| 作者姓名： | 桂亮 张斌 沈健 唐劲草 王猛 封冰 李相成 |
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| 作者单位： | 南京医科大学第一附属医院肝脏外科,210029 |
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| 基金项目： | 江苏省科教兴卫工程(2007200) |
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| 摘 要： | 目的研究远端缺血预处理(RIPC)在小体积肝移植物缺血-再灌注(I-R)损伤中的保护作用。方法将10只大鼠随机分成两组,每组5只,原位肝移植组(A组)和RIPC后肝移植组(B组)。建立30%大鼠肝移植模型,检测术后2、6、12、24 h血清中ALT的水平,RT-PCR、Westernblot分别检测肝脏组织中血红素加氧酶1(HO-1)mRNA和蛋白表达。结果与A组相比,术后B组血清ALT水平下调,HO-1 mRNA及蛋白表达均明显增加(P<0.05)。结论 RIPC具有保护小体积移植物I-R损伤作用,这可能与HO-1在术后肝脏组织中的过表达有关。
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| 关 键 词： | 缺血-再灌注损伤 小体积肝移植 血红素加氧酶1 缺血预处理 |
Protective effect of remote ischemia preconditioning on ischemia-reperfusion injury in a small-for-size liver transplantation model |
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| GUI Liang,ZHANG Bin,SHEN Jian,et al..Protective effect of remote ischemia preconditioning on ischemia-reperfusion injury in a small-for-size liver transplantation model[J].Jiangsu Medical Journal,2011,37(11). |
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| Authors: | GUI Liang ZHANG Bin SHEN Jian |
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| Institution: | GUI Liang,ZHANG Bin,SHEN Jian,et al.Liver Transplantation Center,First Affiliated Hospital,Nanjing Medical University,Nanjing 210029,CHINA |
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| Abstract: | Objective To investigate the protective effect of remote ischemia preconditioning(RIPC) on ischemia-reperfusion (I-R) injury in a small-for-size liver transplantation model.MethodsTen rats were randomly and equally divided into two groups of A(orthotopic liver transplantation) and B(liver transplantation after RIPC).The 30% liver transplantation model in rats was used,and the level of alanine aminotransferase(ALT) was measured at 2,6,12,24h after operation.The expressions of hemeoxygenase-1(HO-1) mRNA and p... |
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| Keywords: | Ischemia-reperfusion injury Small-for-size liver transplantation Hemeoxy-genase-1 Ischemia preconditioning |
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