| 移植预处理后肝脏内皮的电镜制作方法 |
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| 引用本文： | 于红丽,齐昆明,宋国梁,闫志凌,陈翀,董红燕,曾令宇. 移植预处理后肝脏内皮的电镜制作方法[J]. 江苏医药, 2011, 37(13) |
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| 作者姓名： | 于红丽 齐昆明 宋国梁 闫志凌 陈翀 董红燕 曾令宇 |
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| 作者单位： | 1. 徐州医学院神经生物学研究中心,221002
2. 徐州医学院附属医院血液病科移植免疫实验室 |
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| 基金项目： | 国家自然科学基金(81070446) |
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| 摘 要： | 目的介绍一种移植预处理后肝脏内皮的电镜制作方法。方法将30只C57BL/6小鼠随机均分为A组(60Co 7.5 Gy致死量照射)、B组(60Co 5.0 Gy减低剂量照射)和C组(正常对照),通过透射电镜定期观察肝脏内皮变化。结果透射电镜下可见:A组肝脏内皮细胞核碎裂、内皮破坏严重;B组染色质边集,内皮无明显损伤;C组未见明显异常。结论对肝脏组织进行恰当的处理后,透射电镜下能清楚地观察到内皮细胞表面结构和内壁损伤的形态改变。
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| 关 键 词： | 移植预处理 肝脏内皮 透射电镜 |
A method of making electron microscope of the liver endothelium after transplantation conditioning |
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| YU Hongli,QI Kunming,SONG Guoliang,et al.. A method of making electron microscope of the liver endothelium after transplantation conditioning[J]. Jiangsu Medical Journal, 2011, 37(13) |
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| Authors: | YU Hongli QI Kunming SONG Guoliang et al. |
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| Affiliation: | YU Hongli,QI Kunming,SONG Guoliang,et al.Research Center of Neurobiology,Xuzhou Medical College,Xuzhou 221002,CHINA |
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| Abstract: | Objective To introduce a method of making electron microscope of the liver endothelium after the transplantation conditioning.Methods Thirty C57BL/6 mice were randomly and equally divided into three groups of A(exposed to lethal irradiation with 60Co 7.5Gy),B(exposed to reduced irradiation with 60Co 5.0Gy) and C(normal controls).After irradiation,the changes of liver endothelium were regularly observed by transmission electron microscope.Results Under the transmission electron microscope,cell karyorrhexis o... |
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| Keywords: | Transplantation conditioning Liver endothelium Transmission electron microscope |
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