| 靶向Ccnd1基因的siRNA对大鼠晶状体上皮细胞增殖的抑制作用 |
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| 引用本文： | 陆宏,张黎明,管怀进,胡楠.靶向Ccnd1基因的siRNA对大鼠晶状体上皮细胞增殖的抑制作用[J].江苏医药,2011(9). |
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| 作者姓名： | 陆宏 张黎明 管怀进 胡楠 |
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| 作者单位： | 南通大学附属医院眼科; |
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| 摘 要： | 目的探讨靶向Ccnd1基因小干扰RNA(si RNA)对培养的大鼠晶状体上皮细胞(LECs)Ccnd1 mRNA和周期蛋白D1表达及细胞增殖的抑制作用。方法合成3条靶向大鼠Ccnd1基因的siRNA(si-Ccnd1-1,si-Ccnd1-2,si-Ccnd1-3),LipofectamineTM2000转染体外培养的大鼠LECs,应用RT-PCR和Western blot方法分别检测Ccnd1 mRNA和周期蛋白D1表达变化,筛选最有效si RNA序列。MTT法连续测定转染最有效si RNA序列后24、48、72、96和120 h细胞增殖情况。结果与对照组相比,3条si RNA均能不同程度地抑制Ccnd1 mRNA和周期蛋白D1的表达,以si-Ccnd1-3最为有效(P<0.05)。与对照组比较,si-Ccnd1-3转染后24 h开始明显发挥对细胞增殖的抑制作用,并持续至120 h以后(P<0.05)。结论靶向Ccnd1的si RNA能有效降低大鼠LECsCcnd1 mRNA和周期蛋白D1蛋白表达,抑制LECs增殖。
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| 关 键 词： | Ccnd1基因 RNA干扰 晶状体上皮细胞 |
Inhibitory effect of Ccnd1 targeting siRNA on proliferation of lens epithelial cells in rat |
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| LU Hong,ZHANG Liming,GUAN Huaijin,et al..Inhibitory effect of Ccnd1 targeting siRNA on proliferation of lens epithelial cells in rat[J].Jiangsu Medical Journal,2011(9). |
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| Authors: | LU Hong ZHANG Liming GUAN Huaijin |
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| Institution: | LU Hong,ZHANG Liming,GUAN Huaijin,et al.Department of Ophthalmology,Affiliated Hospital,Nantong University,Nantong 226001,CHINA |
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| Abstract: | Objective To evaluate the effect of small interference RNAs(siRNAs) targeting Ccnd1 gene on the expressions of Ccnd1 mRNA and Cyclin D1 and the inhibition of proferation of lens epithelial cells(LECs) in rats.Methods siRNAs targeting Ccnd1 genes(si-Ccnd1-1,si-Ccnd1-2,si-Ccnd1-3) and negative control siRNA were synthesized and transfected into LECs of rat with LipofectamineTM2000 in vitro.The silencing effect of siRNA was determined by quantitative real time-PCR.The expression of Cyclin D1 protein was determ... |
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| Keywords: | Ccnd1 gene RNA interference Lens epithelial cells |
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