Hypermethylation of tumor-related genes in genitourinary cancer cell lines. |
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| Authors: | W B Chung S H Hong J A Kim Y K Sohn B W Kim J W Kim |
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| Institution: | Department of Dental Microbiology, College of Dentistry, Kyungpook National University, Taegu, Korea. jwkim@knu.ac.kr |
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| Abstract: | Hypermethylation of CpG island is a common mechanism for the inactivation of tumor-related genes. In the present study, we analyzed 13 genitourinary cancer cell lines for aberrant DNA methylation of 5 tumor-related genes using methylation- specific polymerase chain reaction (MSP). GSTP1 was methylated in 5 (38.5%), E-cadherin in 1 (8%), VHL in 1 (8%), and MGMT and hMLH1 in none (0%). Six out of thirteen genitourinary cancer cell lines had methylation of at least one of five genes; 5 had one gene methylated, and, 1 had two genes methylated. Methylation of these 5 genes was not detected in any of the bladder cancer cell lines. GSTP1 was methylated in all of the 3 prostate cancer cell lines. We conclude that aberrant hypermethylation may be an important mechanism for the inactivation of cancer-related genes in kidney and prostate cancer cell lines. |
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