商陆皂苷甲对IL-17诱导肾小球系膜细胞增殖的影响

目的:观察商陆皂苷甲(EsA)对IL-17诱导的肾小球系膜细胞(GMC)增殖的影响,探讨EsA对系膜细胞增殖性肾病的作用机制。方法:将GMC分为对照组(不加药物干预),IL-17(5ng/ml)组,IL-17(10ng/ml)组,IL-17(20ng/ml)组,IL-17(50ng/ml)组,IL-17(100ng/ml)组,同步化后分别培养24、48、72h,MTT法检测各组对rGMC增殖的影响,确定IL-17诱导rGMC增殖的作用浓度;将rGMC分为对照组(加入IL-17)、干预组(加入IL-17、终浓度为5mg/LEsA),同步化后分别培养24、48、72h,MTT法检测各组对GMC增殖的影响,ELISA法检测各组细胞上清液中纤维连接蛋白(FN)的表达。结果:IL-17各浓度组均促进了GMC增殖(P<0.05);EsA抑制了IL-17(100ng/ml)诱导的GMC增殖(P<0.05),并下调了FN的表达(P<0.01)。结论:EsA可抑制IL-17诱导的GMC增殖,并减少FN的分泌。

Influence of esculentoside A on the proliferation of IL-17 induced glomerular mesangial cells

Objective:To observe the effect of esculentoside A(EsA)on the proliferation of IL-17 Induced rat glomerular mesangial cells.In order to explore the mechanism of EsA on effects proliferative glomerulonephritis.Methods:The GMC(cell line HBZY-1)divided into control group(only added GMC),IL-17(5 ng/ml)group,IL-17(10 ng/ml)group,IL-17(20 ng/ml)group,IL-17(50 ng/ml)group,IL-17(100 ng/ml)group,with different concentrations of IL-17 stimulation GMC were cultured 24,48,72 h.Detected the proliferation GMC by using MTT assay to determine the IL-17-induced proliferation GMC concentration.The GMC(cell line HBZY-1)divided into control group(added IL-17),the intervention group(IL-17 and adding a final concentration of 5mg/L EsA to intervene),were cultured 24,48,72 h,detected the proliferation GMC by using MTT assay of each group,ELISA method to detect the expression levels of the supernatant fibronectin(FN)of each group.Results:MTT assay the proliferation of IL-17 induced GMC results showed:Compared with the control group,different concentration of IL-17 in each group and were promoted GMC proliferation was statistically significant(P<0.05).MTT assay the intervention of EsA on GMC proliferation induced by IL-17 results showed:the control group,the intervention group suppressed GMC proliferation(P<0.05).ELISA assay the level of FN results showed:FN level of the intervention group was higher than the control group,the difference was statistically significance(P<0.01).Conclusion:EsA can inhibit the proliferation of IL-17 to GMC,and decreased the secretion of FN.