正交试验法优选海南黎药冰糖草茎叶中总黄酮成分的提取工艺

目的:优选了海南黎药冰糖草茎叶中总黄酮成分提取工艺。方法:采用L₁₆(4~5)正交试验,以冰糖草茎叶中总黄酮得率为考察指标,考察乙醇浓度、超声时间、超声温度、超声功率和液料比5个因素对总黄酮得率的影响。结果:海南黎药冰糖草茎叶中总黄酮提取的最佳工艺为80%乙醇浓度、80min超声时间、50℃超声温度、250W超声功率、30∶1ml/g液料比,此条件下总黄酮得率达25.36mg/g。结论:应用该工艺提取黎药冰糖草茎叶中总黄酮,总黄酮得率较高,方便可行,为黎药冰糖草中总黄酮提取工艺的确定提供实验依据。

Optimum selection of the extraction technology of total flavoniods in the leaves and stems of Hainan Li folk medicine scoparia dulcis L.by orthogonal test

Objection:To optimize the extraction technology of total flavoniods in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L.Methods:By orthogonal design L16(45),we changed the ethanol concentration,ultrasonic time,ultrasonic temperature,ultrasonic power and ratio of liquid-to-solid in order to obtain highest extraction rate of total flavonoids.Results:The best technological parameters for extraction of total flavonoids in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L.are as follows:ethanol concentration of 80%,ultrasonic time of 80 min,ultrasonic temperature of 50℃,ultrasonic power of 250W,liquid solid ratio of 30:1ml/g.Under such condition,the yields of total flavonoids were 25.36mg/g.With high extraction yields of total flavonoids,this technologogy is simple and feasible.Conclusion:This study provides experimental basis for extraction of total flavonoids from in the Leaves and Stems of Hainan Li Folk Medicine Scoparia dulcis L.