氦氖激光重复照射对培养瘢痕成纤维细胞凋亡相关基因表达的影响

目的 :从蛋白质水平探讨氦氖激光诱导瘢痕成纤维细胞凋亡的机制。方法 :以波长 6 32 .8nm、功率密度10 0mW /cm2 的氦氖激光照射培养瘢痕成纤维细胞 ,每日 1次 ,每次 30min ,连续照射 3天 ,然后分别采用激光共聚焦扫描显微镜、流式细胞仪测定Bcl- 2、Bax、Fas、ICE、p5 3、c -myc蛋白的细胞分布与表达。 结果 :在培养增生性瘢痕成纤维细胞有多种凋亡相关基因表达蛋白分布 ,氦氖激光照射后 ,Fas、ICE表达增加 ,Bcl- 2表达降低 ,Bax、p5 3、c -myc的表达无变化。结论 :氦氖激光诱导的瘢痕成纤维细胞凋亡与Fas、Bcl- 2、ICE表达蛋白有关

Effects of He-Ne laser repeated irradiation on expression of gene-associated with apoptosis in scar fibroblasts in vitro

Objective:To explore the mechanism of He-Ne laser inducing apoptosis of fibroblasts of cultured hypertrophic scars(HS) at protein level. Method:The cultured fibroblasts in HS were irradiated with He-Ne laser (Wavelength 632.8nm, power density 100mW/cm 2) daily for 30 minutes. After 3 times of He-Ne laser irradiation, the expression of these genes such as Bcl-2, Bax, Fas, ICE, p53 and c-myc were respectively studied by confocal laser scanning microscopy and flow cytometry. Result:Several proteins such as Bcl-2,Fas,ICE,p53 and c-myc proteins were present in scar fibroblasts in culture.The amount of Fas and ICE proteins increased, and Bcl-2 protein reduced,while the amount of Bax, p53 and c-myc proteins remained constant,after repeated He-Ne laser irradiation. Conclusion:He-Ne laser inducing apoptosis of scar fibroblasts were associated with the changes of Fas,ICE and Bcl-2.