| 硫化氢预处理延迟相对大鼠心肌缺血再灌注时谷胱甘肽S转移酶表达的影响 |
| |
| 引用本文： | Ran K,Tang ZG,Ding LP,Li SF,Chang YT. 硫化氢预处理延迟相对大鼠心肌缺血再灌注时谷胱甘肽S转移酶表达的影响[J]. 浙江大学学报(医学版), 2011, 40(5): 535-539. DOI: 10.3785/j.issn.1008-9292.2011.05.012 |
| |
| 作者姓名： | Ran K Tang ZG Ding LP Li SF Chang YT |
| |
| 作者单位： | 1. 中南大学湘雅二医院麻醉科,湖南长沙,410011
2. 长沙市第三医院麻醉科,湖南长沙,410015 |
| |
| 摘 要： | 目的:探讨硫化氢预处理延迟相对大鼠心肌缺血再灌注时谷胱甘肽S转移酶表达的影响.方法:健康成年Sprague-Dawley雄性大鼠40只,随机分成4组:假手术组(S组)仅开胸、分离冠状动脉左前降支,不阻断血流150 min;缺血再灌注组(IR组)行冠状动脉左前降支阻断30 min,再灌注120 min;硫化氢预处理延迟相组(H组)静脉注射硫化氢(0.05 mg/kg),给药后24h同IR组处理;硫化氢预处理延迟相+线粒体KATP通道阻断剂(5-羟葵酸,5-HD)组(D组)缺血前15min静脉注射5-HD 5 mg/kg,其它同H组处理.再灌注结束后测心肌谷胱甘肽S转移酶(GST)的表达和心梗面积,观察心肌细胞超微结构.结果:与IR组(38.27±5.64)%比,H组(25.40±3.54)%心肌梗死面积减小(P＜0.05),D组(40.53±5.24)%无明显差异(P＞0.05).与S组比,IR组、H组和D组GST均升高(P＜0.05),与IR组比,H组GST增高(P＜0.05),D组无明显差异(P＞0.05).与IR组比,电镜下H组心肌细胞损伤程度减轻,D组无明显差异.结论:硫化氢预处理延迟相对大鼠心肌的保护作用可能与上调心肌谷胱甘肽S转移酶表达有关.
|
| 关 键 词： | 心肌 硫化氢/治疗应用 缺血预处理,心肌 谷胱甘肽转移酶 |
Effect of hydrogen sulfide-induced delayed preconditioning on glutathione S-transferase expression during myocardial ischemia-reperfusion in rats |
| |
| Ran Ke,Tang Zheng-guo,Ding Li-ping,Li Shuang-feng,Chang Ye-tian. Effect of hydrogen sulfide-induced delayed preconditioning on glutathione S-transferase expression during myocardial ischemia-reperfusion in rats[J]. Journal of Zhejiang University. Medical sciences, 2011, 40(5): 535-539. DOI: 10.3785/j.issn.1008-9292.2011.05.012 |
| |
| Authors: | Ran Ke Tang Zheng-guo Ding Li-ping Li Shuang-feng Chang Ye-tian |
| |
| Affiliation: | RAN Ke1,TANG Zheng-guo2,DING Li-ping1,LI Shuang-feng1,CHANG Ye-tian1(1.Department of Anesthesiology,Second Xiangya Hospital,Central South University,Changsha 410011,China,2.Department of Anesthesiology,the Third Hospital,Changsha 410015,China) |
| |
| Abstract: | Objective: To investigate the effect of hydrogen sulfide-induced delayed preconditioning on glutathione S-transferase(GST) expression during myocardial ischemia-reperfusion in rats. Methods: Sprague-Dawley male rats were randomly divided into 4 groups(n= 10 in each):Group S(sham operation group),Group IR(ischemia/reperfusion group),Group H(IR+ NaHS 0.05 mg/kg iv,24 h before ischemia) and Groups D receiving IR+NaHS 24 h before ischemia and 5-hydroxydecanoate(5-HD) 15 min before ischemia.Animals in groups IR,... |
| |
| Keywords: | Myocardium Hydrogen sulfide/ther use Ischemic preconditioning myocardial Glutathione transferase |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
