| 特异性下调GRP78基因表达对卵巢癌细胞耐药性的影响 |
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| 引用本文： | 范丽梅,张立会,崔松花,崔满华.特异性下调GRP78基因表达对卵巢癌细胞耐药性的影响[J].吉林大学学报(医学版),2011,37(2):255-259. |
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| 作者姓名： | 范丽梅 张立会 崔松花 崔满华 |
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| 作者单位： | 吉林大学第二医院妇产科,吉林 ,长春 ,130041 |
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| 基金项目： | 吉林省科技厅科技发展计划项目资助课题 |
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| 摘 要： | 目的:探讨RNA干扰技术沉默葡萄糖调节蛋白78（GRP78）基因对人卵巢癌细胞耐药性的影响，阐明GRP78基因沉默逆转肿瘤细胞耐药性的生物学机制。方法：构建pSilencerTM3.0-H1-GRP78 siRNA重组质粒，脂质体介导转染至SKOV3/DDP细胞；RT-PCR和Western blotting法检测GRP78基因的蛋白的表达；Western blotting法检测caspase-4和caspase-3蛋白表达；流式细胞术检测细胞凋亡率。结果：转染GRP78 siRNA重组质粒的SKOV3/DDP细胞GRP78基因和蛋白表达较转染空质粒组明显降低(P<0.05)；加用顺铂后，转染GRP78 siRNA重组质粒细胞组较未转染GRP78 siRNA重组质粒细胞组caspase-4和caspase-3表达明显增加(P<0.05)，细胞凋亡率也明显增加(P<0.05)。结论：抑制GRP78基因表达能通过上调caspase-4和caspase-3表达及增加顺铂诱导的SKOV3/DDP细胞凋亡率，降低SKOV3/DDP细胞对顺铂的耐药性。
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| 关 键 词： | 葡萄糖调节蛋白78 卵巢肿瘤 耐药性 细胞凋亡 |
| 收稿时间： | 2010-08-25 |
Effect of GRP78 gene expression down-regulation on cisplatin-resistance of human ovarian cancer SKOV3/DDP cells |
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| FAN Li-mei,ZHANG Li-hui,CUI Song-hua,CUI Man-hua.Effect of GRP78 gene expression down-regulation on cisplatin-resistance of human ovarian cancer SKOV3/DDP cells[J].Journal of Jilin University: Med Ed,2011,37(2):255-259. |
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| Authors: | FAN Li-mei ZHANG Li-hui CUI Song-hua CUI Man-hua |
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| Institution: | Department of Gynecology and Obstetrics,Second Hospital,Jilin University,Changchun 130041,China |
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| Abstract: | Objective To investigate the effect of siRNA interfering glucose regulated protein78(GRP 78) on cisplatin-resistance of human ovarian cancer SKOV3/DDP cells,and clarify the biomechanism of the effect of GRP78 gene silencing on the cisplatin-resistance of human ovarian cancer SKOV3/DDP cells.Methods The pSilencerTM3.0-H1-GRP78 siRNA plasmid was constructed,then it was transfected into cancer cells by Lipofectamine 2 000.The expressions of GRP78 mRNA and protein were measured by RT-PCR and Western blotting.Th... |
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| Keywords: | glucose regulated protein78 ovarian neoplasms drug resistance apoptosis |
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