| 低剂量电离辐射诱导小鼠睾丸中细胞色素c和caspase-3表达的变化 |
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| 引用本文： | 方芳,刘扬,吕喆,杨筱茜,赵红光,龚守良,王志成.低剂量电离辐射诱导小鼠睾丸中细胞色素c和caspase-3表达的变化[J].吉林大学学报(医学版),2011,37(2):191-195. |
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| 作者姓名： | 方芳 刘扬 吕喆 杨筱茜 赵红光 龚守良 王志成 |
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| 作者单位： | 吉林大学公共卫生学院营养与食品卫生学教研室,吉林,长春,130021;吉林大学公共卫生学院,卫生部放射生物学重点实验室,吉林,长春,130021;吉林大学公共卫生学院,卫生部放射生物学重点实验室,吉林,长春,130021;中国医科大学附属第一医院心血管内科,辽宁,沈阳,110000;吉林大学第一医院核医学科,吉林,长春,130021 |
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| 基金项目： | 国家自然科学基金课题，吉林大学基本科研业务费项目资助课题 |
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| 摘 要： | 目的:研究低剂量电离辐射诱导小鼠睾丸中细胞色素c(Cyt c)和caspase-3表达的变化,阐明二者对低剂量电离辐射诱导小鼠睾丸细胞凋亡的作用.方法:实验小鼠分为不同剂量(0、0.050、0.075、0.100和0.200 Gy)组与不同时程(0、6、12和24 h)组.采用实时PCR和Western blottin...
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| 关 键 词： | 细胞色素c caspase-3 实时PCR 电离辐射 生精细胞 小鼠 |
| 收稿时间： | 2010-11-06 |
Changes of Cyt c and caspase-3 expressions in mouse testis induced by low dose irradiation |
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| FANG Fang,LIU Yang,LU Zhe,YANG Xiao-xi,ZHAO Hong-guang,GONG Shou-liang,WANG Zhi-cheng.Changes of Cyt c and caspase-3 expressions in mouse testis induced by low dose irradiation[J].Journal of Jilin University: Med Ed,2011,37(2):191-195. |
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| Authors: | FANG Fang LIU Yang LU Zhe YANG Xiao-xi ZHAO Hong-guang GONG Shou-liang WANG Zhi-cheng |
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| Institution: | 1.Department of Nutrition and Food Hygiene,School of Public Health,Jilin University,Changchun 130021,China;2. Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021| China;3.Department of Cardiology,First Affiliated Hospital,China Medical University,Shenyang 11000,China;4. Department of Nuclear Medicine,First Hospital,Jilin University,Changchun 130021|China） |
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| Abstract: | Objective To investigate the effect of low dose irradiation on the expression changes of Cyt c and caspase-3 in mouse testis,and interpret the effects on apoptosis in mouse testis induced by irradiation.Methods The mice were divided into different doses(0,0.050,0.075,0.100 and 0.200 Gy)groups and different time course(0,6,12 and 24 h) groups.The expressions of Cyt c and caspase-3 mRNA and proteins in mouse testis tissues irradiated with different doses X-rays were observed by real time PCR and Western blott... |
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| Keywords: | caspase-3 |
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