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Targeted superparamagnetic nanoparticles coated with 2-deoxy-d-gloucose and doxorubicin more sensitize breast cancer cells to ionizing radiation
Institution:1. Department of Medical Physic, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;2. Research Center for Pharmaceutical Nanotechnology, Research and Development Complex, Tabriz University of Medical Sciences, Tabriz, Iran;3. Department of Medicinal Chemistry, School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran;4. Department of Radiology and Radiotherapy, Imam Reza Medical Center for Treatment and Training, Tabriz, Iran;5. Department of Statistics & Epidemiology, School of Health & Nutrition, Tabriz University of Medical Sciences, Tabriz, Iran;1. Tianjin Key Laboratory of Biomaterials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, PR China;2. Department of Biomedical Engineering, University of Minnesota, 7-116 Hasselmo Hall, 312 Church Street S.E, Minneapolis, MN 55455, USA;1. Chemistry Department, K.Maras Sütcü Imam University, 46100 K.Maras, Turkey;2. Chemistry Department, Çank?r? Karatekin University, 18100 Çank?r?, Turkey;1. Instituto de Investigaciones Biomédicas, Universidad de las Américas, Quito – Ecuador;2. Grupo Investigación Identificación Genética IdentiGEN, Universidad de Antioquia, Medellín – Colombia;3. Laboratorio de Genética Molecular, Cruz Vital - Cruz Roja Ecuatoriana, Quito – Ecuador;4. Grupo de Investigación de Biotecnología. Instituto de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medellín – Colombia;5. Laboratorio de Genética, Instituto Nacional de Medicina Legal y Ciencias Forenses, Bogotá – Colombia;1. Radiation Oncology Unit, Azienda Ospedaliero-Universitaria Careggi, University of Florence, Italy;2. Cancer Risk Factors and Lifestyle Epidemiology Unit, Cancer Research and Prevention Institute (ISPO), Italy;3. Breast Unit, S. Maria Annunziata Hospital, Florence, Italy;4. Diagnostic Senology Unit, Azienda Ospedaliero-Universitaria Careggi, Italy;5. Struttura Complessa di Senologia Clinica, Cancer Research and Prevention Institute (ISPO), Italy;6. Department of Surgery, Azienda Ospedaliero-Universitaria Careggi, University of Florence, Italy;7. Division of Pathological Anatomy, Department of Medical and Surgical Critical Care, University of Florence, Florence, Italy;1. Department of Applied Physics, University of Eastern Finland, 70211 Kuopio, Finland;2. Department of Applied Physics, Aalto University, 02150 Espoo, Finland;3. Department of Physics and Astronomy, University of Turku, 20014 University of Turku, Finland;4. A.I. Virtanen Institute for Molecular Science, University of Eastern Finland, 70211 Kuopio, Finland;3. Cell Death, Senescence, and Survival Group, Departament de Bioquímica i Biologia Molecular and Institut de Neurociències, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Barcelona, Spain;4. Cell Signalling and Apoptosis Group, Vall d''Hebron-Institut de Recerca, 08035 Barcelona, Spain;5. Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), 08035 Barcelona, Spain
Abstract:IntroductionNanoparticles are promising as a new approach to enhance chemo- radiotherapy efficiency in breast cancer mainly via targeted therapy.Materials & methodsSKBR3 and T47D breast cancer cells were treated with superparamagnetic mesoporous hydroxyapatite nanocomposites (SPmHANs)conjugated with 1 μM doxorubicin and 0.5 mM 2-Deoxy-d-Glucose and irradiated with 1 and 2 Gy gamma rays in vitro. The treatment toxicity and also the apoptosis/necrosis ratio were measured by MTT assay and also ELISA cell death detection PLUS, respectively.ResultsThe decreased cell viability with the combined treatment, with determined 42% loading efficiency for 200 ppm 2DG and 93% for5ppm doxorubicin on SPmHANs in PH about 7.4 and 5.5, were calculated to 60.9% and 68% compared to radiotherapy alone inT47D and SKBR3 cells (both with p < 0.05), respectively.ConclusionBreast cancer cure may boost from The combined targeted nanoparticle treatment with doxorubicin and 2-Deoxy-d-Glucose may boost breast cancer radiotherapy by improved chemodrug localization, increased cytotoxicity in tumor cells and decreased single modality treatment doses.
Keywords:Breast cancer  Chemotherapy  Doxorubicin  Nanocomposite  Ionizing radiation  2DG"}  {"#name":"keyword"  "$":{"id":"kwrd0045"}  "$$":[{"#name":"text"  "$$":[{"#name":"__text__"  "_":"Two-Deoxy-"}  {"#name":"small-caps"  "_":"d"}  {"#name":"__text__"  "_":"-Glucose  ATP"}  {"#name":"keyword"  "$":{"id":"kwrd0055"}  "$$":[{"#name":"text"  "_":"Adenosine triphosphate  CT"}  {"#name":"keyword"  "$":{"id":"kwrd0065"}  "$$":[{"#name":"text"  "_":"Computed tomography  DOX"}  {"#name":"keyword"  "$":{"id":"kwrd0075"}  "$$":[{"#name":"text"  "_":"Doxorubicin  HA"}  {"#name":"keyword"  "$":{"id":"kwrd0085"}  "$$":[{"#name":"text"  "$$":[{"#name":"italic"  "_":"hydroxyapatite  MNP"}  {"#name":"keyword"  "$":{"id":"kwrd0095"}  "$$":[{"#name":"text"  "_":"Magnetic nanoparticle  MRI"}  {"#name":"keyword"  "$":{"id":"kwrd0105"}  "$$":[{"#name":"text"  "_":"Magnetic resonance imaging  PET"}  {"#name":"keyword"  "$":{"id":"kwrd0115"}  "$$":[{"#name":"text"  "_":"Positron emission tomography  SPION"}  {"#name":"keyword"  "$":{"id":"kwrd0125"}  "$$":[{"#name":"text"  "_":"Superparamagnetic iron oxide nanoparticle
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