Lack of endothelial autophagy does not impair liver regeneration after partial hepatectomy in mice |
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| Authors: | Genís Campreciós Aina Anton Anna Oncins Carla Montironi Maria Ruart Rosa Montañés Héctor García-Calderó Joan Carles García-Pagán Virginia Hernández-Gea |
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| Affiliation: | 1. Barcelona Hepatic Hemodynamic Laboratory, Liver Unit, Hospital Clínic, Health Care Provider of the European Reference Network on Rare Liver Disorders (ERN-Liver), Barcelona, Spain;2. Pathology Department and Molecular Biology Core, Centre de Diagnòstic Biomèdic (CDB), Hospital Clínic de Barcelona, Universitat de Barcelona, Barcelona, Spain |
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| Abstract: | Liver sinusoidal endothelial cells (LSEC) are key elements in regulating the liver response to injury and regeneration. While endothelial autophagy is essential to protect endothelial cells from injury-induced oxidative stress and fibrosis, its role in liver regeneration has not been elucidated. This study was intended to investigate the role of endothelial autophagy in liver regeneration in the context of partial hepatectomy (PHx). Analysis of autophagy levels in rat LSEC after PHx indicated a tendency to decrease activity the first 2 days after surgery. PHx performed in mice with impaired endothelial autophagy (Atg7flox/flox;VE-Cadherin-Cre+) and their littermate controls showed no differences neither in liver-to-body weight ratio, histological analysis, hepatocyte proliferation nor vascular integrity during the first 7 days after PH and liver regeneration was completely achieved. Our results indicate that endothelial autophagy does not play an essential role in the coordination of the liver regeneration process after PHx. |
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| Keywords: | endothelial autophagy liver regeneration LSEC oxidative stress partial hepatectomy |
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