A rabbit model of aneurysmal subarachnoid hemorrhage by ear central artery-suprasellar cistern shunt |
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| Affiliation: | 1. Department of Neurosurgery, Longyan First Hospital Affiliated to Fujian Medical University, Longyan, Fujian 364000, China;2. Department of Neurosurgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, China;3. Department of Neurology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, China;1. Kyoto Institute of Nutrition and Pathology Inc, Kyoto, Faculty of Pharmaceutical Science, Setsunan University, Japan;2. Masa-no-Mori Pet Clinic, Kanasawa, Faculty of Pharmaceutical Science, Setsunan University, Japan;3. Laboratory of Pathology, Faculty of Pharmaceutical Science, Setsunan University, Neyagawa, Japan;1. Université Claude Bernard Lyon 1, Lyon, France;2. Groupe ELSAN, Clinique Bretéché, Nantes, France;3. Hôpital Pierre Wertheimer, Hospices Civils de Lyon, Lyon, France;1. The Jiangsu Key Laboratory on Opto-Electronic Technology, School of Physical Science and Technology, Nanjing Normal University, China;2. Patent Examination Cooperation Jiangsu Center of the Patent Office, SIPO, China;1. INESC TEC and Department of Physics and Astronomy, Faculty of Sciences of University of Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal;2. Wroclaw University of Science and Technology, Faculty of Fundamental Problems of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland;3. Maria Curie-Sklodowska University, Laboratory of Optical Fibre Technology, Pl. M. Curie-Sklodowskiej 3, 20-031 Lublin, Poland;1. College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China;2. State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, China;3. Xinjiang Uygur Autonomous Region Animal Husbandry General Station, Urumqi, Xinjiang, 830001, China;4. Institute Veterinary, Animal & Biomedical Sciences, Massey University, Auckland, Palmerston North, New Zealand;5. First Affiliated Hospital, School of Medical College, Shihezi University, Shihezi, Xinjiang, 832008, China |
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| Abstract: | Aneurysmal subarachnoid hemorrhage (aSAH) is a life-threatening hemorrhagic cerebrovascular disease. The concept of early brain injury (EBI), induced by sharply increased intracranial pressure (ICP) and low cerebral perfusion pressure (CPP) with cerebral global ischemia following aneurysm rupture, has been increasingly accepted. However, EBI has not been well studied partly due to lack of an ideal animal model. The purpose of this study was to establish a new aSAH model which can mimic the pathophysiological damage of EBI. Right frontal craniotomy was performed on New Zealand rabbits for placing a PE-50 tube at the suprasella cistern and an ICP probe at the anterior cranial fossa. The central ear artery was punctured and blood was shunted into the suprasellar cistern through the PE-50 tube. ICP, blood pressure, CPP and heart rate peri-aSAH were monitored throughout the experiments. The rabbits were examined for neurological deficits at 24 h post-SAH. Brain coronal sections near the optic chiasma were assessed by HE and Cresyl violet staining. Three minutes after SAH induction, the ICP peaked to 61.7 ± 9.8 mmHg while CPP decreased to nadir 23.5 ± 8.9 mmHg, and both were gradually restored in 15 min. At 24 h post-SAH, significant neurological deficits were found in SAH rabbits as compared to the sham-operated animals. In addition, neuronal degeneration and loss were also detected. Our results indicate that a new rabbit model of aSAH with EBI is successfully established. Moreover, this model is controllable, economical, and no side-injury to the main artery. |
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| Keywords: | Aneurysmal subarachnoid hemorrhage Animal model Early brain injury Rabbit |
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