基质金属蛋白酶及其抑制因子与神经母细胞瘤侵袭转移的关系

目的　探讨基质金属蛋白酶 (MMP) 2、MMP 9及其抑制因子 (TIMP) 2、TIMP 1与神经母细胞瘤侵袭转移的关系。方法　应用免疫组化SABC法检测 35例神经母细胞瘤标本中MMP 2、MMP 9及TIMP 2、TIMP 1的表达情况 ,并比较其在无转移、局部转移及远处转移患者肿瘤组织中的表达程度。结果　在有局部转移的B组和远处转移的C组患儿中 ,MMP 2高表达者分别为 9例和 10例 ,相对于病灶局限的A组明显升高 (P <0 .0 5 )。MMP 9表达仅在A组和C组间有统计学意义 (P <0 .0 5 )。预后良好的A组TIMP 2的阳性表达程度最高 ,随着肿瘤进展表达程度逐渐减弱。各组间TIMP 1表达差异无统计学意义 (P =0 .36 5 2 )。结论　MMP 2、MMP 9的表达与神经母细胞瘤的侵袭转移有关 ,可促进肿瘤进展 ,而TIMP 2则抑制肿瘤细胞的侵袭转移。

Correlation between expression of MMP-2, MMP-9, TIMP-2, TIMP-1 and metastasis of neuroblastoma

Objective To study MMP 2, MMP 9, TIMP 2 and TIMP 1 expression, and their association to invasion and metastasis of neuroblastoma (NB). Methods The staining status was compared of MMP 2, MMP 9, TIMP 2 and TIMP 1 in cryostat sections of tumor tissue in 35 NB patients by immunohistochemistry. Results Strong expression of MMP 2 was detected only in 2 patients with early stage NB (group A without metastasis), but in 9 and 10 respectively with advanced stage NB (group B with local metastasis and group C with distant metastasis) (compared to group A, P <0.01). Strong MMP 9 staining was found in 4, 8 and 11 patients for group A, B and C patients (group A vs group C, P <0.05). The expression of TIMP 2 was the strongest in 4 group A patients,but it decreased with progression of the disease. There was no statistical difference in TIMP 1 expression among the three groups of patients. Conclusion MMP 2?MMP 9 expression may be related to metastasis and progression of neuroblastoma, while TIMP 2 may have an inhibitory effect.