左旋多巴用量与帕金森病异动症的实验研究

目的观察由不同用量左旋多巴诱发的帕金森病（PD）大鼠模型异动症（LID）的行为学区别，探讨左旋多巴用量与模型大鼠LID表现之间的关系。方法选择通过6-羟基多巴（6-OHDA）由右侧中脑前脑束（MBF）二点注射法成功制备的PD大鼠模型．随机分成左旋多巴甲酯10mg／kg体重用药组和50mg／kg体重用药组，行药物腹腔注射，2次／d，连续21d，比较不自主动作（AIM）评分和旋转行为。结果总共28只雌性SD大鼠中22只成功制备PD模型，造模成功率78．57％；左旋多巴二种用量虽均可诱发模型大鼠LID，但表现有差异；2组实验大鼠在用药后出现AIM时间、AIM持续时间、AIM评分均值、药效期及药效期内的旋转行为等方面存在差异，有统计学意义（P〈0．05）。结论LID严重程度与左旋多巴用量有关；左旋多巴用量大，则出现重症LID的比例高。

Experimental research of levodopa dosage and levodopa-induced dyskinesia

Objective To ohaerve the behavior of PD rat models treated with different dosages of levodopa and analyze the relation- ship between levodopa - induced dyskinesia (LID) and dosage of levedopa. Method The successful PD rat models were set up by 6-OHDA injection into right MFB. Two groups were divided according to the different treatment dosages of levodopa, which were given through perito- neal injection twice a day for 21days. One group was given for 10 mg/kg body weight, the other for 50 mg/ kg body weight. The score of AIM and rotational behavior were observed. Results Among altogether 28 female SD rats, 22 were chosen as the successful PD models and the successful rate arrived at 78. 57%. LID could be induced in both groups, but there were great differences in occurrence and duration time of AIM, AIM score, pharmacodynamic action time, and rotational behavior between these two groups( P<0.05). Conclusions The be- havior of LID has great relationship with the given dosage of levodopa.