肝硬化大鼠睾丸一氧化氮合酶免疫组化研究

探讨肝硬化睾丸功能障碍的发生机制.将清洁级雄性SD大鼠分为假手术组(SO,n=10)、肝硬化组(CIR,n=10).采用胆管结扎(BDL)复制肝硬化模型,假手术组则仅分离出胆总管而不予结扎.应用免疫组织化学法对SO组和CIR组大鼠睾丸组织内皮型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)的表达进行比较性研究.结果术后4周,组织学检查显示胆汁性肝硬化特征性表现.CIR组和SO组大鼠睾丸组织eNOS表达均在间质细胞胞浆,二者平均吸光度和面密度相比较均无显著性差异(P＞0.05).而iNOS表达则存在较大差异,SO组仅在睾丸组织间质细胞胞浆表达;CIR组不仅在间质细胞胞浆,还在支持细胞、生精细胞以及脱落的生精上皮细胞胞浆表达,二者平均吸光度及面密度相比均存在非常显著性差异(P＜0.01).表明CIR组大鼠睾丸组织iNOS活性增高,参与了支持细胞、生精过程的调节,从而进一步证明了肝硬化睾丸功能障碍的发生机制中存在NO途径.

Immunohistochemical Study of NOS in Testicular Tissue of Cirrhotic Rats

The pathogenesis of testicular dysfunction in cirrhotic rats was investigated. Twenty SD rats were divided into two groups equally: cirrhotic group and sham operated group. Cirrhotic rat model was induced by bile duct ligation (BDL). The expression of endothelial NOS (eNOS) and inducible NOS (iNOS) in testicular tissue was detected by using immunohistochemisty and compared between the two groups. Results showed that after 4 weeks, the histological examination showed billiary cirrhosis. The expression of eNOS distributed in cytoplasm of leydig cells of testicular tissue in both two groups. There was no significant difference in average optical density and area density of eNOS between the two groups ( P >0 05). However, there was significant difference in iNOS expression between the two groups. iNOS was expressed only in the cytoplasm of leydig cells of testicular tissue in the sham operated group, but in the cirrhotic group, iNOS expression of testicular tissue was detectable in the cytoplasm of sertoli cells, germ cells and sloughed germ cells besides the cytoplasm of leydig cells. There was significant difference in average optical density and the area density of iNOS between the two groups. It was demonstrated that iNOS activities were increased in testicular tissue of the cirrhotic rats and iNOS was abnormally expressed in the cytoplasm of sertoli cells and germ cells, suggesting that NO acts on soterli cells and spermateleosis and is associated with testicular dysfunction in cirrhosis.