TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway |
| |
Authors: | Wei Yu Jieping Huang Hui Yu Jingping Lin Fang Fan Ruixiang Xie Yongshi Shen Kangni Lin Yong Ye Jinsen Weng |
| |
Affiliation: | 1.Department of Clinical Pharmacy, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China;2.Emergency Department, Fujian Medical University Union Hospital, Fuzhou, China;3.Department of Critical Care Medicine, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China |
| |
Abstract: | BackgroundThe aim of this study was to investigate the inhibiting effect of transient receptor potential vanilloid 3 (TRPV3) on the proliferation and migration of colorectal cancer (CRC) cells and to explore the underlying mechanism.MethodsA microarray dataset from the publicly available Gene Expression Omnibus (GEO) database was used to investigate the prognostic value of TRPV3 in CRC. In addition, 100 CRC tissue samples were collected at our center to further validate its prognostic value at the protein level. Cell proliferation ability was detected by Cell Counting Kit-8 (CCK-8) assay, and cell migration ability was detected by transwell assay. Gene set variation analysis (GSVA) was performed to identify the potential pathways regulated by TRPV3.ResultsBased on the largest microarray dataset ({"type":"entrez-geo","attrs":{"text":"GSE39582","term_id":"39582"}}GSE39582), low expression of TRPV3 was found to be significantly associated with poor prognosis in CRC patients, and this result was successfully validated at our cancer center. Functional experiments showed that knockdown of TRPV3 enhanced cell proliferation and migration, while enforced TRPV3 expression exhibited the opposite effect. GSEA based on public microarray data revealed that the mitogen-activated protein kinase (MAPK) signaling pathway was notably activated in patients with low expression of TRPV3. Further experiments in vivo confirmed that TRPV3 silencing promoted cell proliferation and migration by activating the MAPK signaling pathway.ConclusionsLow expression of TRPV3, which stimulates cell proliferation and migration by provoking the MAPK signaling pathway, indicated poor prognosis in CRC patients. |
| |
Keywords: | Colorectal cancer (CRC) transient receptor potential vanilloid 3 (TRPV3) cell proliferation and migration mitogen-activated protein kinase signaling pathway (MAPK signaling pathway) |
|
|