Testosterone-stimulation of adenyl cyclase and cyclic 3',5'-adnosine monophosphate- 3 H formation in rat seminal vesicles

Seminal vesicles obtained from normal or castrate rats were capable of converting radioactive adenosine into labeled cyclic 3′,5′-adenosine monophosphate (cAMP-³H). Addition of testosterone (10^?6M) in vitro caused about a 60 per cent stimulation in the levels of vesicular cAMP-³H. Castration (96 hr) led to a significant decrease in the content of cAMP-³H and adenyl cyclase activity of this accessory organ; it did not significantly decrease the concentration. A single injection of testosterone to castrate rats produced a marked enhancement of vesicular adenyl cyclase (96 hr later). These results support the concept that stimulation of the cAMP-adenyl cyclase system is associated with the mechanism of action of androgens on their target cells.