HPLC法同时测定复合氨基酸多维胶囊中L-茶氨酸和维生素B_6的含量

目的建立同时测定复合氨基酸多维胶囊中L-茶氨酸和维生素B6含量的HPLC法。方法采用Phmemex LunaC18柱,以0.005 mol/l的十二烷基硫酸钠溶液(pH3.0)-乙腈(66:34)为流动相,流速1.0 ml/min,检测波长205 nm。结果专属性良好,L-茶氨酸、维生素B6、空白辅料与其他功效成分以及其酸、碱、氧化破坏产物无干扰。L-茶氨酸在64.19～96.29μg/ml的范围内线性关系良好,γ=0.9994,回收率为101.1%,中间精密度、重复性良好。维生素B6在8.10～12.15μg/ml范围内呈良好线性,γ=0.9993,回收率为100.1%,中间精密度、重复性良好。结论该反向高效液相色谱法能有效分离检测本胶囊中L-茶氨酸和维生素B6的含量。

DETERMINATION OF L-THEANINE AND PYRIDOXAL IN CAPSULE OF COMPOUND AMINO ACIDS AND VITAMINS BY HPLC

Objective To develop a method of detecting the content of L-theanine and pyridoxal simaltaneously in capsule of compound amino acids and vitamins.Method A C18 column was used with the mobile phase of 0.005mol/L sodium dodecyl sulfate(adjusted to PH3.0 with phosphoric acid)-acetonitrile(34:66) at the detection wavelength of 205 nm.The flow rate was 1.0ml/min.Results The specificity was good.These was no interference of the products after acid or soda treatment or oxidation of L-theanine and pyridoxal and blank accessories and other effective compositions.L-theanine showed linearity from 64.19-96.29 μg/ml,r=0.9994.The rate of reclaimation was 101.1%.Both reproducibility and intermedial precision were good.And pyridoxal showed linearity from 8.10-12.15 μg/ml,r=0.9993.The rate of reclaimation was 100.1%.Both reproducibility and intermediary precision were good.Conclusion This method can separate and detect L-theanine and pyridoxal of the same capsule effectively.