杭州地区男性素食者平均血小板体积与血浆磷脂脂肪酸构成及维生素B_(12)等生化指标的相关研究

目的研究杭州地区男性素食者平均血小板体积(meam platelet volume,MPV)与血浆磷脂脂肪酸构成、血清维生素B12(vitamin B12,VB12)及同型半胱氨酸(homocysteine,Hcy)等指标的相关性。方法采用气相色谱法测定89例男性素食者和103例对照组的血浆磷脂脂肪酸的构成,采用常规方法测定MPV和血清生化指标。结果与对照组相比,素食组血浆多不饱和脂肪酸(n-3 PUFA)水平降低,而n-6 PUFA水平升高;素食组血清VB12水平显著降低(160.7±64.3 vs 373.9±160.9,P<0.01),Hcy水平显著增高,约为对照组的1.74倍(23.74±12.66 vs 13.62±9.17,P<0.01);素食组MPV高于对照组(9.14±1.04 vs 8.82±1.00,P<0.05)。相关性分析显示,MPV与22:5n-6(DPA n-6)呈正相关,与血清VB12水平呈负相关。结论杭州地区男性素食者MPV增高,血清Hcy升高,VB12与n-3 PUFA摄入不足,补充VB12与n-3 PUFA可能对降低素食者的MPV和Hcy有益。

MEAN PLATELET VOLUME, PLASMA PHOSPHOLIPID FATTY ACID COMPOSITIONS AND VITAMIN B12 IN HANGZHOU MALE LACTO-VEGETARIANS AND OMNIVORES

Objective To assess relationship between meam platelet volume(MPV) and plasma phospholipid fatty acid compositions and analyze routine item such as serum vitamin B12(VB12) and homocysteine(Hcy) in Hangzhou male lacto-vegetarians and omnivores.Method Eighty-three lacto-vegetarians and 103 omnivores were recruited.Plasma phospholipid fatty acid compositions were analyzed by gas chromatography.MPV and serum index were tested by routine method.Results Plasma n-3 poly-unsaturated fatty acids(PUFA) of vegetarians were significantly lower and n-6 PUFA were significantly higher than those of controls,serum vitamin B12 level of vegetarian was only about half of omnivores(160.7±64.3 vs 373.9±160.9,P<0.01) while serum Hcy was about twice of the controls(23.74±12.66 vs 13.62±9.17,P<0.01),MPV of vegetarians was significantly higher than controls(9.14±1.04 vs 8.82±1.00,P<0.05),MPV was significantly positively correlated with n-6 PUFA docosapentaenoic acid(22:5n-6;DPA) and negatively correlated with serum VB12.Conclusion Ingestion of VB12 and n-3 PUFA was deficient for male lacto-vegetarians in HangZhou and vegetarians had higher MPV and serum Hcy.The results suggest VB12 and n-3 PUFA fortifed food may be helpful to ruduce MPV and serum Hcy for vegetarians.