民族补益药藏波罗花根几种提取物的抗氧化活性和果蝇延寿作用

目的：研究藏波罗花的抗氧化、抗衰老活性价值，并探索此类活性强度随提取部位极性差异的变化规律。方法：制备不同极性的五种提取物，通过多酚含量测定、LPO抑制和自由基清楚实验评价体外抗氧化活性及变化规律；通过果蝇寿命实验评价体内延寿活性及变化规律。结果：五种提取物的总多酚含量具有溶剂依赖性，由高到低顺序为：IYS4≥IYSI〉〉IYS3〉IYS5〉IYS2．稍亲脂段（IYS1和IYS4）具有优良的LPO抑制活性和O2-、·OH清除活性（以维生素c、苯甲酸、BHT为对照），而亲水段（IYS2、IYS3和IYS5）的活性不明显。提取物的体外抗氧化活性强弱顺序为：IYS4〉IYS1〉IYS3〉IYS52IYS2。用稍亲脂段IYS1或IYS4（饲料中药物终浓度5．0mgmL0）喂养果蝇，与空白相比，IYS1、IYS4可分别使雌果蝇的平均寿命延长24．4％和23．0％，可分别使雄果蝇的平均寿命延长15．3％和16．9％。IYSl、IYS4可分别使雌果蝇的最高寿命延长8．4％和11．2％，可分别使雄果蝇的最高寿命延长9．7％和15．8％。与空白相比，IYSl或IYS4喂养的雌雄果蝇的生存曲线（15天后）都非常显著地向右移动。用亲水段IYS2、IYS3或IYS5（5．0mg·mL-1）喂养的果蝇，延寿作用不明显。各提取物的果蝇体内延寿作用也具有一定的溶剂依赖性，活性高低顺序为：IYS4〉IYS1〉〉IYS3〉IYS2〉IYS5。结论：五种提取物具有优良体外抗氧化活性和显著的果蝇体内延寿活性，且在体外抗氧化活性与多酚含量之间存在正相关关系，延寿活性与多酚含量（或体外抗氧化活性）间也存在一定的正相关关系。各提取物对雌果蝇的延寿作用一般优于对雄果蝇延寿的作用。

Extracts from the roots of Incarvillea younghusbandii on antioxidant effects and life span prolonging in Drosophila melanogaster

AIM： To investigate antioxidant activities and life span prolonging effects of the extracts from the roots oflncarvillea younghusbandii Sprague, and to study the correlations between these activities and the polar intensity of the extracts. METHOD： Five extracts （IYS1, IYS2, IYS3, IYS4 and YSS） with different polar intensity were prepared. Antioxidant activities in vitro were determined by LPO inhibitory and free radicals scavenging experiments. Life span prolonging effects in vivo were evaluated by feeding Drosophila melanogaster. RESULT： Total phenolic content in extracts were solvent-dependent and decreased in the order of IYS4 〉 IYS1 〉〉 IYS3 〉 IYS5 〉 IYS2. Organic extracts （IYS1 and IYS4） showed excellent LPO inhibitory activity, O2＂-and .OH scavenging activity compared to ascorbic acid （or benzoic acid, or BHT）, while aqueous extracts （IYS2, IYS3 and IYS5） did not. The antioxidant activities （in vitro） were solvent dependent and decreased in the order ofIYS4 〉 IYS1 〉 IYS3 〉 IYS5 〉_ IYS2. Drosophila melanogaster was fed with organic extracts （IYS1 or IYS4） at 5.0 mg mL-1. The mean life span were increased by 24.4% （IYS1） or 23.0% （IYS4） in female and 15.3% （IYSI） or 16.9% （IYS4） in male; the maximum life span were increased by 8.4% （[YSI） or 11.2% （IYS4） in female and 9.7% （IYS1） or 15.8% （IYS4） in male, and the survival curves were significantly shifted to the right after fifteen days in both sexes survival period. Feeding aqueous extracts （IYS2, IYS3 or IYS5） at 5.0 mg/mL-1, the significant life span prolonging effects were not achieved. The life span prolonging effects of the extracts were solvent-dependent and decreased in the order of IYS4 〉 IYS1 〉〉 IYS3 〉 IYS2 〉 IYS5. CONCLUSION： Extracts from the roots of Incarvillea younghusbandii Sprague showed excellent antioxidant activities and significant life span prolonging effects in Drosophila melanogaster. Positive correlations existed between the antioxidant activities and total phenolic content. Life span prolonging effect was positively correlated with the total phenolic content or antioxidant activities. The extracts possess better life span prolonging effect in females than in males.