Role of P-selectin and anti-P-selectin monoclonal antibody in apoptosis during hepatic/renal ischemia reperfusion injury |
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Authors: | Wu Pei Li Xiao Zhou Tong Zhang Ming-Jun Chen Jin-Lian Wang Wei-Ming Chen Nan Dong De-Chang |
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Affiliation: | 1. Department of Nephrology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China 2. Animal Laboratory, Ruijin Hospital, Shanghai Second Medical University 3. Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University |
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Abstract: | AIM To evaluale the potential role of P-selectin and anti-P-selectin monoclonal antibody (mAb) in apoptosis during hepatic/renal ischemiareperfusion injury. METHODS Plasma P-selectin level, hepatic/renal P-selectin expression and cell apoptosis were detected in rat model of hepatic/ renal ischemia-reperfusion injury. ELISA, immunohistochemistry and TUNEL were used. Some ischemia-reperfusion rats were treated with antiP-selectin mAb. RESULTS Hepatic/ renal function insufficiency, up-regulated expression of P-selectin in plasma and hepatic/renal tissue, hepatic/renal histopathological damages and cell apoptosis were found in rats with hepatic/renal ischemiareperfusion injury, while these changes became less conspicuous in animals treated with anti-P selectin mAb. CONCLUSION P-selectin might mediate neutrophil infiltration and cell apoptosis and contribute to hepatic/renal ischemia-reperfusion injury, anti-P-selectin mAb might be an efficient approach for the prevention and treatment of hepatic/renal ischemia-reperfusion injury. |
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Keywords: | hepatic ischemia reperfusion injury renal ischemia reperfusion injury P-selectin antibody,monoclonal apoptosis |
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