| RBC花环法识别精液中T淋巴细胞的研究 |
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| 引用本文： | 张军,马远方,赵粤萍,徐志喜,白慧玲,许国强,杜耀武.RBC花环法识别精液中T淋巴细胞的研究[J].河南大学学报(医学版),2002,21(1):12. |
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| 作者姓名： | 张军 马远方 赵粤萍 徐志喜 白慧玲 许国强 杜耀武 |
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| 作者单位： | 河南大学,分子免疫学实验室,河南,开封,475001 |
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| 基金项目： | 河南省教育厅资助课题 (2 0 0 0 310 0 14 ) |
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| 摘 要： | 目的 :研究一种识别精液中T淋巴细胞的方法。方法 :以 96例男性不育症门诊病人为测试对象 ,用冻干抗人T淋巴细胞单克隆抗体致敏的RBC与处理后的精液混合 ,置于显微镜下观察结果。结果 :凡粘附 3个以上致敏RBC者为淋巴细胞 ,未粘附RBC者为生精细胞。结论 :用RBC花环法识别精液中的T淋巴细胞可以作为一种切实可行的方法在临床上应用
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| 关 键 词： | 致敏RBC 淋巴细胞 生精细胞 |
| 文章编号： | 1008-2867(2002)01-0012-01 |
| 修稿时间： | 2001年5月29日 |
Detecting the T Lymphocytes in Semen with the Method of sRBC Rosette |
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| ZHANG Jun,MA Yuan_fang,ZHAO Yue_ping,et al.Detecting the T Lymphocytes in Semen with the Method of sRBC Rosette[J].Journal of Henan University,2002,21(1):12. |
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| Authors: | ZHANG Jun MA Yuan_fang ZHAO Yue_ping |
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| Abstract: | Objective: To study the method of detecting the T lymphocytes in semen.Methods: 96 samples of infertility patients were checked by mixing up semen with red bolld cell snsitized by ice_drjed human T lymphocyte monoclone antibody, and observed through a microscope.Results: The cells clinged with more than 3 sRBCs were T lymphocytes unile non_clinged ones were androgones.Conclusion: The method of detecting the T lymphocytes in semen with SRBC is very effective, can be applied in clinic. |
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| Keywords: | SRBC lymphocyte androgone |
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