乙肝两对半乳胶层析检测试剂板试用评价 |
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引用本文: | 熊杰,白生华,马义.乙肝两对半乳胶层析检测试剂板试用评价[J].西南军医,2003,5(6):13-14. |
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作者姓名: | 熊杰 白生华 马义 |
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作者单位: | 1. 成都军区总医院检验科,成都,610083 2. 解放军第47医院药剂科,四川新都,610500 |
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摘 要: | 目的 评价乙肝两对半乳胶层析检测试剂板。方法 采用酶联免疫吸附法和乳胶层析试剂板对定值血清和88份临床标本作平行检测,结果作对比分析。结果 乳胶测试板对乙肝两对丰的检测限分别达到HBsAg 1ng/ml,HBsAb 30mIu/ml,HBeAg 1NCU/ml,HBeAb 4NCU/ml,HBeAb 2NCU/ml,特异性采用夹心法的三项达到100%,其余两项为94.9%和93.9%,与酶联法相当。溶血对乳酸层检析测无影响。结论 乳胶层析检测试剂板操作简便、快速,结果可靠,适用于单个或急诊病人的快速检测。
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关 键 词: | 乙肝两对半 乳胶层析检测 溶血 |
修稿时间: | 2003年8月27日 |
Evaluation of Emulsoid Immunochromatography Assay for Antigen-antibody System of Hepatitis B |
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Authors: | XIONG Jie BAI Sheng-hua MA Yi |
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Abstract: | Objective To evaluate the commercial stripe of emulsoid immunochromatography assay (EICA) for antigen-antibody system of Hepatitis B. Methods Several fixed value and 88patients samples were detected for antigen-antibody system of Hepatitis B with ELISA and the strip of EICA simultaneously. Results With EICA the detected reached HBsAg Ing/ml,HBsAb 30mIu/ml,HBeAg INCU/ml.HBeAb 4NCU/ml,HBcAb 2NCU/ml,and their specificities were 100% (HBsAg,HBeAg,HBsAb), 94.9% (HBeAb)and 93.9% (HBcAb) .The above mentioned parameters paralleled ELISA.Hematolysis didn't afifect the results of EICA. Conclusions The commercial stripe of EICA is easy, reliable in application and the result can be gained rapidly; it is suitable in detecting respective samples,or emergency samples. |
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Keywords: | emulsoid immunochlomatography assay (EICA) Antigen-antibody system of Hepatitis B Hematolysis |
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