| 一种新的小胶质细胞分离纯化方法 |
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| 引用本文： | 项正兵,吴晓牧,丁伟荣,张昆南,柳喆,何丹.一种新的小胶质细胞分离纯化方法[J].江西医学院学报,2007,47(2):13-14,19. |
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| 作者姓名： | 项正兵 吴晓牧 丁伟荣 张昆南 柳喆 何丹 |
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| 作者单位： | [1]江西省人民医院神经内科,南昌330006 [2]江西省人民医院临床医学研究所,南昌330006 |
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| 摘 要： | 目的 建立一种高效率的原代小胶质细胞体外分离纯化方法.方法 以McCarthy等经典的小胶质细胞原代培养培养方法为基础,并进行改进,使用低浓度胰酶消化法帮助小胶质细胞从混合培养的胶质细胞分离下来,用OX42抗体免疫组化染色鉴定.结果 获得了高纯度的小胶质细胞.结论 低浓度胰酶消化法分离小胶质细胞与经典的振摇分离法所获得的纯度相当,但提高了细胞的分离效率.
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| 关 键 词： | 小胶质细胞 培养 分离 鉴定 小胶质细胞 分离纯化方法 Cell Identification Method Isolation 分离效率 分离法 高纯度 结果 免疫组化染色 抗体 细胞分离 混合培养 胰酶消化法 浓度 使用 改进 培养方法 细胞原代培养 McCarthy |
| 文章编号： | 1000-2294（2007）02-0013-02 |
| 修稿时间： | 2007-01-10 |
A Innovative Microglia Isolation Method and Cell Identification |
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| XIANG Zheng-bing,WU Xiao-mu,DING Wei-rong,ZHANG Kun-nan,LIU Zhe,HE Dan.A Innovative Microglia Isolation Method and Cell Identification[J].Acta Academiae Medicinae Jiangxi,2007,47(2):13-14,19. |
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| Authors: | XIANG Zheng-bing WU Xiao-mu DING Wei-rong ZHANG Kun-nan LIU Zhe HE Dan |
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| Abstract: | Objective To establish a method of improved efficient primary mouse microglia isolation and its purification.Methods Primary microglia culture was modified based on the classic microglia culture method of McCarthy's. Trypsinization was employed with 0.05% trypsin for the isolation of the microglia from mixed cultured cells. Microglial cells were identified by immunohistochemistry using mouse anti rat CD11b monoclonal antibody(OX42).Results High purity of Primary microglial cell was obtained.Conclusion The purity of microglia by the method of mild trypsinization was equal to that of McCarthy's. but the efficiency of microglia isolation is improved. |
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| Keywords: | microglia culture isolation identification |
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