桂产藿香蓟水提物抗炎作用及其机制研究

目的：探讨桂产藿香蓟水提物的抗炎作用及其机制。方法将60只小鼠随机分为5组：空白对照组、地塞米松组、桂产藿香蓟水提物高、中、低剂量组,采用二甲苯诱导小鼠耳廓肿胀,观察桂产藿香蓟水提物对二甲苯诱导小鼠耳廓肿胀的影响；以同样的分组方法另取60只小鼠,采用角叉菜胶致小鼠足肿胀法,观察桂产藿香蓟水提物对角叉菜胶致小鼠足肿胀的影响,并分别测定小鼠足肿胀中丙二醛、前列腺素E2和超氧化物歧化酶的活性。实验结果组间比较采用单因素方差分析,两两组间对比采用 LSD 检验。结果与空白对照组比较,桂产藿香蓟水提物高、中、低剂量组能显著抑制小鼠耳廓肿胀和足肿胀,抑制率分别为29．27%、18．23%、11．61%和27．58%、17．15%、7．93%,差异具有统计学意义（P＆lt;0．01）；空白对照组前列腺素E2、丙二醛和超氧化物歧化酶含量分别为124．95±15．71μg/g、21．19±3．27 nmol/ml、162．19±12．52 U/ml,桂产藿香蓟水提物其高、中、低剂量组前列腺素E2、丙二醛和超氧化物歧化酶含量分别为（52．12±9．51μg/g、78．30±11．60μg/g、83．41±13．34μg/g ）、（14．57±2．51 nmol/ml、15．40±2．34 nmol/ml、16．37±3．18 nmol/ml ）及（222．76±19．59 U/ml、209．90±15．30 U/ml、194．64±19．68 U/ml）,与空白对照组比较,桂产藿香蓟水提物其高、中、低剂量组能显著降低小鼠炎足中炎性组织前列腺素E2、丙二醛含量,提高其超氧化物歧化酶活力,差异具有统计学意义（ P〈0．01）。结论桂产藿香蓟水提物抗炎作用显著,其机制可能与降低炎性组织中前列腺素E2的含量,提高超氧化物歧化酶活性、清除氧自由基有关。

Study on anti-inflammatory effects and its mechanisms of water extract of Ageratum conyzoides L. from Guangxi

Objective To explore anti-inflammatory effects and its mechanisms of water extract of Ageratum conyzoides. L. from Guangxi. Methods 60 mice were randomly divided into five groups：control group, dexamethasone group, water extract of Ageratum conyzoides. L. from Guangxi with high, medium and low dosage group, then induce auricle edema by dimethylbenzene to investigate the anti-inflammatory effect of water extracts of Ageratum conyzoides. L. from Guangxi on auricle edema. Using the same grouping meth-od, induce paw edema by carrageenan to investigate the anti-inflammatory effect of water extracts of Agera-tum conyzoides. L. from Guangxi, and measure the content of malondiadehyde（MDA）, prostaglandin E2 （ PGE2 ） and the activity of superoxide dismutase （ SOD ） in edema paw. Difference comparison among groups was determined with single factor variance analysis. Comparison between two groups was performed with LSD test. Results Compared with the control group, the water extracts of Ageratum conyzoides. L. from Guangxi with high, medium and low dosage groups could remarkably inhibit the ear edema and the paw edema in mice, the inhibition ratio were 29. 27%, 18. 23%, 11. 61% and 27. 58%, 17. 15%,＆amp;nbsp;7. 93% respectively. The difference had statistical significance（P＆lt;0. 01）. The content of MDA, PGE2 and SOD in the edema paw in the control group were 124. 95 ± 15. 71 μg/g, 21. 19 ± 3. 27 nmol/ml and 162. 19 ± 12. 52 U/ml respectively. The high, medium and low dosage group were （ 52. 12 ± 9. 51μg/g, 78. 30 ± 11. 60 μg/g and 83. 41 ± 13. 34 μg/g）,（14. 57 ± 2. 51 nmol/ml, 15. 40 ± 2. 34 nmol/ml and 16. 37 ± 3. 18 nmol/ml）and（222. 76 ± 19. 59 U/ml, 209. 90 ± 15. 30 U/ml and 194. 64 ± 19. 68 U/ml）re-spectively. Compared with control group, it could remarkably reduce MDA and PGE2 production and en-hanced activity of SOD in mice inflammatory tissue, the difference had statistical significance （P〈0. 01）. Conclusion The water extracts of Ageratum conyzoides. L. from Guangxi has significantly anti-inflammato-ry effects, and its mechanisms may relate to its ability of reducing the content of MDA and PGE2 , enhan-cing the activity of SOD, and scavenging oxygen free radicals in mice inflammatory tissue.