广西虎纹捕鸟蛛毒成分的氨基酸测定及红外光谱定性分析

目的通过质量分析的方法初步鉴定蛛毒的毒素化学成分。方法采用氨基酸自动分析仪对蛛毒的多种氨基酸成分进行定性及定量的研究,并且辅以红外光谱法进行定性研究。结果通过氨基酸测定法从虎纹捕鸟蛛中鉴定到谷氨酸、胱氨酸、赖氨酸含量较多,分别为8.15%、7.86%、7.64%,氨基酸总量为55.93%;从样品粉末的红外光谱可知：该化合物可能含-C-H,-OH,NH,-CO-N-H,苯环等主要氨基酸基团。结论结果表明该鉴别方法操作简便、专属性强,可用于蛛毒的定性鉴别和含量测定,初步建立了虎纹蛛毒成分的定性鉴定研究方法。

Amino acid assay of toxin from the spider Guangxi Selecnocosmia Huwena and qualitative analysis by infrared spectrometry

Objective Preliminary identification of the chemical composition of toxin from the spider Guangxi Selenocosmia Huwena with the method of quality analysis. Methods Automatic amino acid analyzer on a variety of amino acid composition of spider poisons was adopted to perform a qualitative as well as quantitative research,and the former was supplemented by infrared spectrometry. Results By using amino acids analysis the chemical composition of Guangxi Selenocosmia Huwena toxin was identified, and the finding was the spider Guangxi Selenocosmia Huwena contains higher levels of glutamic acid,cystine,lysine,which were 8. 15%,7. 86%,and 7. 64%,respectively,and the total amino acids was 55. 93%. The infrared spectrometry showed that the sample powder may contain-C-H,-O-H,N-H,-CO-N-H, benzene ring and other major amino group. Conclusion The results showed that the identification method, being simple,and specific,could be used for spider poison qualitative identification and determination, and the specific approaches for such method was tentatively established.