反相高效液相色谱法测定人血浆中非诺贝特活性代谢物非诺贝酸

目的:建立以高效液相色谱法测定人血浆中非诺贝特活性代谢物非诺贝酸的方法。方法:血浆样品酸化后用乙醚萃取;色谱柱为Shim-pack VP-ODS(150mm×4．6mm),柱温为40℃,流动相为甲醇-水-10％磷酸(80:19:1),流速为1．0mL·min-1,检测波长为290nm,外标法峰高定量。结果:非诺贝酸的保留时间约为5．6min,定量线性范围为0．25～18．75μg·mL-1,绝对回收率>85％(n=5),方法回收率为96％～103％(n=5),日内、日间RSD<10％(n=5)。结论:本方法简便快速、定量准确,适用于非诺贝特临床药动学研究。

Determination of Fenofibric Acid in Human Plasma by RP - HPLC

OBJECTIVE:To develop an RP- HPLC method to determine fenofibric acid in plasma. METHODS:The plasma samples were extracted with diethyl ether after acidification.The chromatographic column was Shim- pack VP-ODS column (150mm×4.6mm) .The column temperature was 40℃.The mobile phase consisted of methanol - water - 10% phosphoric acid (80:19:1),with the flow rate of 1.0mL·min-1 and the detective wavelength was set at 290nm.RESULTS:The retention time of fenofibric acid was 5.6min.The standard curve were linear for fenofibric acid in the range of 0.25-18.75μg·mL-1. The absolute recovery was greater than 85% (n = 5) ,and the method recovery was 96%-103% (n = 5) .The intra-day and inter-day RSD were less than 10% (n = 5) .CONCLUSION: The proposed method is convenient, sensitive and reliable, and can be used for pharmacokinetic studies of fenofibrate.