同种异体组织工程化软骨组织形成的初步研究

目的 探讨同种异体组织工程化软骨在动物体内形成。方法 取怀孕１００ｄ胚胎羊的关节软骨经体外消化分离软骨细胞，与可吸收生物材料Ｐｌｕｒｏｎｉｃ Ｆ１２７混合形成细胞－生物材料复合物，将细胞－生物材料复合物注射到异体羊腹壁皮下，每隔２周取材，连续半年对所取标本进行大体观察、称重，并作ＨＥ、Ｓａｆｒａｎｉｎ Ｏ、Ｍａｓｓｏｎ’ｓ ｔｒｉｃｈｒｏｍｅ染色，对再生的软骨组织进行评价。结果 在同种异体动物体内

An initial experiment and study on allogenic tissue engineered cartilage

OBJECTIVE: To investigate the formation of engineered cartilage in vivo with allogenic chondrocytes. METHODS AND MATERIALS: Joint cartilage from ewe embryos (Pregnancy: 100 days) was isolated and digested to obtain dissociated chondrocytes. Mixed chondrocytes with biomaterials (Pluronic 127) at the density 50 x 10(6)/ml, then implant the allograft complex subcutaneously. Collect the novel tissue every two weeks along for six months, weigh and stain (HE, Safranin O, Masson's trichrome) the sample tissues individually to evaluate the characteristics of novel tissue. RESULT: Novel cartilage can regenerate in allograft animals, with similar histological properties of chondrocytes, aminoglycin and collagen distribution to normal cartilage. The novel cartilage observed histologically showed apparent surrounding inflammatory cells in two and four week and the tissue of cartilage came to mature since the sixth week, with less inflammatory reaction, but not disappeared. CONCLUSION: Novel cartilage can generate in allograft animals with tissue engineering approach, with certain immunoreaction surrounding. Such reaction will be weakened gradually with time.