尿脱落细胞端粒酶活性检测在膀胱癌中的诊断价值

目的：探讨通过检测尿脱落细胞端粒酶活性早期诊断膀胱癌。方法：采用改进的银染－TRAP（端粒重复序列扩增）法，分别对45例膀胱移行细胞癌患者，10例膀胱良性病变患者及8例正常人尿脱落细胞进行了端粒酶活性检测。结果：24例G1级（分化良好）的膀胱癌患者，端粒酶活性强阳性12例，弱阳性6例（阳性率75．0％），21例G2，G3级膀胱癌患者，强阳性16例，弱阳性3例（阳性率94．5％），膀胱良性病变患者及正常人尿脱落细胞只有1例端粒酶活性呈弱阳性，其余全部阴性。结论：尿脱落细胞端粒酶活性检测特异性强，敏感性高，与病理分类，组织分级等无明显关系，提示有可能成为膀胱癌临床早期诊断指标之一。

Detection of telomerase activity in exfoliated rotheliated cells of bladder cancer and its clinic value

ve:To explore the diagnosis value of the telomerase activity of exfoliated rothelial cells of bladder cancer. Methods: Combining silver stain with TRAP, we detected the telomerase activity in exfoliated rothelial cells of 45 cases of bladder cancer and 10 normal bladder tissues and 8 non - rothelial cancer. Results: Among 45 malignant exfoliated rothelial cells which were diagnosed by pathology, telomerase activity was strong positive in 28 and weak positive in 9(37/45). Telomerase activity was weak positive in 1 case of 12 negative controls. Conclusions: There is a close relationship between telomerase activity and rine samples of bladder cancer patients. Telomerase will be one of the marks for diagnosis of bladder cancer.