| A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies |
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| 作者姓名： | Zhankui Liu Shengshun Tan Chunshui Yu Jinghua Fan Zhuanli Bai Junjie Li |
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| 作者单位： | Department of Dermatology, the Second Affiliated Hospital, Medical School of Xi＇an Jiaotong University, Xi＇an 710004. Shanxi Province, China |
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| 摘 要： | Objective：To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods：Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons. Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results ：Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients, while nine other different proteins expressing from healthy volunteers. Conclusion：The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics.
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| 关 键 词： | 银屑病 皮肤病 牛皮癣 发病机制 |
| 收稿时间： | 2007/2/26 0:00:00 |
| 修稿时间： | 2007-02-26 |
A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies |
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| Zhankui Liu,Shengshun Tan,Chunshui Yu,Jinghua Fan,Zhuanli Bai,Junjie Li.A preliminary screening study on the associated proteins in human psoriasis vulgaris by serum proteomics technologies[J].Acta Universitatis Medicinalis Nanjing,2007,21(4):272-276. |
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| Authors: | Zhankui Liu Shengshun Tan Chunshui Yu Jinghua Fan Zhuanli Bai and Junjie Li |
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| Abstract: | Objective:To investigate the optimum screening conditions of associated proteins in human psoriasis vulgaris by serum proteomics technique, and to screen the different expression proteins related with psoriasis vulgaris. Methods:Serum samples of peripheral blood were collected from newly diagnosed psoriasis vulgaris patients in the clinic, and 20 matched healthy persons.Serum albumin IgG was removed by filtering with ProteoExtract Albumin/IgG. After comparative proteomics analysis the different protein spots were identified using 2-DE and MS. Results :Electrophoresis figures with high resolution and reproducibility were obtained. Three different expression proteins were found only in the serum from psoriasis vulgaris patients,while nine other different proteins expressing from healthy volunteers. Conclusion:The protein expression was different in the serum between the psoriasis vulgaris patients and healthy volunteers. It was hoped that we could find the biomarkers related to psoriasis vulgaris by using proteomics. |
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| Keywords: | electrophoresis gel psoriasis vulgaris proteomics biological technologies proteomics serum psoriasis vulgaris human proteins associated study find biomarkers protein expression healthy volunteers Electrophoresis figures high resolution reproducibility Results analysis filtering Albumin |
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