| Culture and purification of human fetal olfactory ensheathing cells using different attachment rates combined with intermittent NT3 nutrition |
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| 作者姓名： | Qiang Li Xijing He Guozhou Rao Pei Fan Bin Wang |
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| 作者单位： | [1]Department of Orthopedics, the Second Affiliated Hospital of Xi＇ an Jiaotong University, Xi＇ an 710004, shanxi province, China [2]Hospital of Stomatology, Xi＇ an Jiaotong University, Xi＇ an 710004, shanxi province, China |
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| 基金项目： | This work was supported by the Special Foundation for Doctoral Discipline of Education Ministry （20050698043） |
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| 摘 要： | Objective：To explore a simple and pragmatic method to obtain sufficient olfactory ensheathing cells from human fetus by selective attachment of harvested cells combined with intermittent NT3 nutrition. Methods：DMEM/F12 culture solution including 10% fetal bovine serum or NT3 was used to culture olfactory ensheathing cells intermittently every 48 h. The cell state and growth rates of OECs were observed, and P75 staining was used to estimate the purity of the cells. Results：Human fetal OECs were positive with P75 immunocytochemical staining. OECs in dipolar or tripolar shape formed networks by their processes in vitro. The purity of OECs in ＂good state＂ was about 95% at 9 d and 83% on 12 d, respectively. Conclusion：The method of using different attachment rates combined with intermittent NT3 addition is a simple and effective way to culture and purify OECs.
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| 关 键 词： | 神经细胞 嗅觉细胞 细胞培养 NT3 |
| 收稿时间： | 2007/5/22 0:00:00 |
| 修稿时间： | 2007-05-22 |
Culture and purification of human fetal olfactory ensheathing cells using different attachment rates combined with intermittent NT3 nutrition |
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| Qiang Li,Xijing He,Guozhou Rao,Pei Fan,Bin Wang.Culture and purification of human fetal olfactory ensheathing cells using different attachment rates combined with intermittent NT3 nutrition[J].Acta Universitatis Medicinalis Nanjing,2007,21(5):307-310. |
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| Authors: | Qiang Li Xijing He Guozhou Rao Pei Fan and Bin Wang |
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| Abstract: | Objective:To explore a simple and pragmatic method to obtain sufficient olfactory ensheathing cells from human fetus by selective attachment of harvested cells combined with intermittent NT3 nutrition. Methods:DMEM/F12 culture solution including 10% fetal bovine serum or NT3 was used to culture olfactory ensheathing cells intermittently every 48 h. The cell state and growth rates of OECs were observed, and P75 staining was used to estimate the purity of the cells. Results:Human fetal OECs were positive with P75 immunocytochemical staining. OECs in dipolar or tripolar shape formed networks by their processes in vitro. The purity of OECs in "good state" was about 95% at 9 d and 83% on 12 d, respectively. Conclusion:The method of using different attachment rates combined with intermittent NT3 addition is a simple and effective way to culture and purify OECs. |
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| Keywords: | olfactory ensheathing cell cell culture purification NT3 nutrition combined growth rates attachment different olfactory ensheathing cells human purification method of addition effective purify good shape networks processes in vitro Human positive immunocytochemical staining |
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