| Study of apoptosis pattern of dopaminergic neurons and neuroprotective effect of nicotine in MPTP mouse model |
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| 引用本文： | Dan Hu,Wei Cao,Shenggang Sun. Study of apoptosis pattern of dopaminergic neurons and neuroprotective effect of nicotine in MPTP mouse model[J]. 南京医科大学学报(自然科学版), 2007, 21(4): 248-252 |
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| 作者姓名： | Dan Hu Wei Cao Shenggang Sun |
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| 作者单位： | [1]Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hukei Province, China [2]Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hukei Province, China |
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| 摘 要： | Objective：To investigate the apoptosis of dopaminergic neurons and the protective effect of nicotine in 1-methyl-4-phenyl- 1, 2,3,6-tetrahydropyridine （MPTP）-induced mouse model of Parkinson＇s disease. Methods：The mouse model of Parkinson＇s disease were formed by MPTP （30 mg/kg/d×7, i.p. ）; and the loss and apoptosis of dopaminergic neurons was observed by Tyrosine Hydroxylase（TH） and TUNEL stains.In ＂Nicotime plus MPTP＂ group, mice were pretreated with nicotine before MPTP injection. The putative protective effect of nicotine was analyzed. Results：The number of TH-positive cells decreased during MPTP treatment. Apoptotic neurons began to appear after three injections of MPTP and peaked on the 8th day. In the MPTP-intoxicated mice treated with nicotine, the loss of TH-positive cells was significantly less than that of MPTP- treated group （30 mg/kg/d×7）（P 〈 0.05）. Conclusion：The chronic treatment of MPTP can induce the apoptosis of dopaminergic neurons in substantia nigra, and nicotine might have a neuroprotecitve effect on dopaminergic neurons against MPTP toxicity.
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| 关 键 词： | 帕金森疾病 细胞凋亡 多巴胺能神经元 烟碱 |
| 收稿时间： | 2007-02-26 |
| 修稿时间： | 2007-02-26 |
Study of apoptosis pattern of dopaminergic neurons and neuroprotective effect of nicotine in MPTP mouse model |
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| Dan Hu,Wei Cao and Shenggang Sun. Study of apoptosis pattern of dopaminergic neurons and neuroprotective effect of nicotine in MPTP mouse model[J]. Acta Universitatis Medicinalis Nanjing, 2007, 21(4): 248-252 |
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| Authors: | Dan Hu Wei Cao Shenggang Sun |
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| Affiliation: | Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, Hubei Province, China;Department of Nuclear Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, Hubei Province, China;Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan 430022, Hubei Province, China |
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| Abstract: | Objective:To investigate the apoptosis of dopaminergic neurons and the protective effect of nicotine in 1-methyl-4-phenyl-1, 2,3,6-tetrahydropyridine (MPTP)-induced mouse model of Parkinson's disease. Methods :The mouse model of Parkinson's disease were formed by MPTP (30 mg/kg/d×7, i.p.); and the loss and apoptosis of dopaminergic neurons was observed by Tyrosine Hydroxylase (TH) and TUNEL stains. In "Nicotime plus MPTP" group, mice were pretreated with nicotine before MPTP injection. The putative protective effect of nicotine was analyzed. Results:The number of TH-positive cells decreased during MPTP treatment. Apoptotic neurons began to appear after three injections of MPTP and peaked on the 8th day.In the MPTP-intoxicated mice treated with nicotine, the loss of TH-positive cells was significantly less than that of MPTP-treated group (30 mg/kg/d×7)(P ＜ 0.05). Conclusion:The chronic treatment of MPTP can induce the apoptosis of dopaminergic neurons in substantia nigra, and nicotine might have a neuroprotecitve effect on dopaminergic neurons against MPTP toxicity. |
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| Keywords: | Parkinson's Disease apoptosis 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine Nicotine mouse model MPTP nicotine neuroprotective effect dopaminergic neurons pattern apoptosis toxicity chronic substantia nigra appear number cells during treatment Results mice injection group plus |
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