甘薯糖蛋白对动脉粥样硬化家兔血脂和一氧化氮影响的实验研究

目的:观察甘薯糖蛋白对实验性动脉粥样硬化家兔血脂和一氧化氮的影响并探讨其可能的发病机制.方法:将40只新西兰雄兔随机分为正常对照组(饲以普通饲料)、高脂组(饲以普通饲料 胆固醇)、低剂量甘薯糖蛋白组(饲以普通饲料 胆固醇 糖蛋白0.06g/kg.d)和高剂量甘薯糖蛋白组(饲以普通饲料 胆固醇 糖蛋白0.1g/kg.d).在实验开始时和12周时分别检测血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇以及血清中一氧化氮含量.第12周末处死动物,行主动脉粥样硬化病理形态学观察.结果:实验前各组血清检测指标无明显差异.实验12周,高脂组、低剂量甘薯糖蛋白组和高剂量甘薯糖蛋白组血清总胆固醇、甘油三酯、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇以及一氧化氮含量均明显高于正常对照组(p<0.05或p<0.01),但低剂量甘薯糖蛋白组和高剂量甘薯糖蛋白组的上述指标除高密度脂蛋白胆固醇升高外,其它的都明显低于高脂组(p<0.01);同时低剂量甘薯糖蛋白组和高剂量甘薯糖蛋白组动物主动脉斑块面积明显小于高脂组(p<0.05或p<0.01).结论:甘薯糖蛋白对动脉粥样硬化有明显的预防和治疗作用,可能与其降血脂及减少一氧化氮生成有关,并且这种作用具有一定的量效关系.

RESERCH ON EFFECT OF BATATA GLYCOPROTEIN ON BIOOD LIPIDS AND NO IN ATHEROCLERETIC RABBITS

Objective:To observe the effect of batata glycoprotein(BG) on blood lipids and NO in atheroclderetic rabbits and its mechanisms. Methods:Fourty adult male New-Zealand rabbits were randomly divided into normal control group, hyper-cholesterol group, low dose BG and high dose BG group. Each groups were fed respectively with common forage, high cholesterol forage, high cholesterol forage adding BG(0.06g/kg.d) and high cholesterol forage adding BG(0.1g/kg.d ). At week 0 and 12,the levels of total cholesterol(TC), triglyceride (TG),low density lipoprotein cholesterol(LDLC) and high density lipoprotein cholesterol(HDLC) in the serum were all determined. The levels of serum nitric oxide (NO) were measured. At the end of the twelfth week, all rabbits were killed to observe aorta pathologic changes. Results:At the end of the twelfth week, the levels of serum TC,TG,LDLCand NO were significantly decreased in low dose group and high dose group compared with those in hypercholesterol group(p<0.05or p<0.01), while the levels of serum HDLC were significantly increased in low dose group and high dose group compared with those in hypercholesterol group(p<0.01). Plaque area of high dose group was smaller than that of hypercholesterol group (p<0.05or p<0.01). Conclusion:Batata glycoprotein can effectively decrease the levels of blood lipids and NO , and the effect correlates with the amount of adding batata glycoprotein. Batata glycoprotein is a potential drug in treating and preventing atherosclerosis.