| 人4-1BBL基因重组腺病毒载体的构建及其在HEK293细胞的表达 |
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| 引用本文： | 杨明花,王帅,田昕,王扬,隋承光. 人4-1BBL基因重组腺病毒载体的构建及其在HEK293细胞的表达[J]. 解剖科学进展, 2011, 17(5): 445-449 |
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| 作者姓名： | 杨明花 王帅 田昕 王扬 隋承光 |
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| 作者单位： | 中国医科大学附属第一医院肿瘤研究所,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省科技厅科学技术计划项目 |
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| 摘 要： | 目的 体外构建含有人4-IBBL基因的重组腺病毒载体,并检测其在HEK293细胞中的表达.方法 设计一对含有Sfil酶切位点的4-1BBL因上下游引物,以质粒pCR4-TOP0-4-1BBL为模板,通过PCR扩增获得4-1BBL基因全序列.片段回收后经酶切处理,连接到穿梭质粒pShuttle-CMV-EGFP上,获得重...
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| 关 键 词： | 4-1BBL 腺病毒 基因表达 共刺激分子 |
Construction and expression of human 4-1BBL recombinant adenovirus vector in HEK293 cells |
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| YANG Ming-hua,WANG Shuai,TIAN Xin,WANG Yang,SUI Cheng-guang. Construction and expression of human 4-1BBL recombinant adenovirus vector in HEK293 cells[J]. Progress of Anatomical Sciences, 2011, 17(5): 445-449 |
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| Authors: | YANG Ming-hua WANG Shuai TIAN Xin WANG Yang SUI Cheng-guang |
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| Affiliation: | YANG Ming-hua,WANG Shuai,TIAN Xin,WANG Yang,SUI Cheng-guang* (Cancer Research Institute,First Affiliated Hospital,China Medical University,Shenyang 110001 China) |
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| Abstract: | Objective To construct the recombinant adenovirus vector contained human 4-1BBL gene and to check the target gene expression in HEK293 cells. Methods Primer containing SfiI was designed and full length 4-1BBL cDNA was obtained from the pCR4-TOPO-4-1BBL plasmid by PCR, the PCR product was digested with restrictive endonucleases SfiI, then inserted directionally into pShuttle-CMV-EGFP. The plasmid of pShuttle-EGFP-4-1BBL was lined with SfiiI, the fragment containing 4-1BBL was reclaimed and then transfected i... |
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| Keywords: | 4-1BBL adenovirus gene expression costimulating molecule |
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